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Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology
文献类型：期刊论文
| 作者 | Li, C; Bai, JH; Cai, ZL; Fan, OY |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2002-01-31 |
| 卷号 | 93期号:1页码:27-34 |
| 关键词 | bacteriocin Lactococcus lactis response surface methodology optimization |
| ISSN号 | 0168-1656 |
| 其他题名 | J. Biotechnol. |
| 中文摘要 | The medium composition for bacteriocin production by Lactococcus lactis ATCC 11454 was optimized using response surface methodology. The selected six factors based on CM medium were sucrose, soybean peptone, yeast extract, KH2PO4, NaCl, and MgSO4.7H(2)O. Fractional factorial designs (FFD) and the path of steepest ascent were effective in searching for the main factors and approaching the optimum region of the response. By a 2(6-2) FFD, sucrose, soybean peptone, yeast extract, KH2PO4 were found to be significant factors and had positive effects on cell growth, however, only soybean peptone and KH2PO4 were shown to be the two significant factors for bacteriocin production and had negative and positive effects, respectively. The effects of the two main factors on bacteriocin production were further investigated by a central composite design and the optimum composition was found to be 1% sucrose, 0.45% soybean peptone, 1% yeast extract, 2.84% KH2PO4, 0.2% NaCl, and 0.02% MgSO4.7H(2)O. The optimal medium allowed bacteriocin yield to be doubled compared to CM medium. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | The medium composition for bacteriocin production by Lactococcus lactis ATCC 11454 was optimized using response surface methodology. The selected six factors based on CM medium were sucrose, soybean peptone, yeast extract, KH2PO4, NaCl, and MgSO4.7H(2)O. Fractional factorial designs (FFD) and the path of steepest ascent were effective in searching for the main factors and approaching the optimum region of the response. By a 2(6-2) FFD, sucrose, soybean peptone, yeast extract, KH2PO4 were found to be significant factors and had positive effects on cell growth, however, only soybean peptone and KH2PO4 were shown to be the two significant factors for bacteriocin production and had negative and positive effects, respectively. The effects of the two main factors on bacteriocin production were further investigated by a central composite design and the optimum composition was found to be 1% sucrose, 0.45% soybean peptone, 1% yeast extract, 2.84% KH2PO4, 0.2% NaCl, and 0.02% MgSO4.7H(2)O. The optimal medium allowed bacteriocin yield to be doubled compared to CM medium. (C) 2002 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | NISIN PRODUCTION ; BATCH FERMENTATIONS ; COMPLEX |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000173186700003 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5610]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, C,Bai, JH,Cai, ZL,et al. Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology[J]. JOURNAL OF BIOTECHNOLOGY,2002,93(1):27-34. |
| APA | Li, C,Bai, JH,Cai, ZL,&Fan, OY.(2002).Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology.JOURNAL OF BIOTECHNOLOGY,93(1),27-34. |
| MLA | Li, C,et al."Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology".JOURNAL OF BIOTECHNOLOGY 93.1(2002):27-34. |
入库方式： OAI收割
来源：过程工程研究所
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