Refolding human lysozyme produced as an inclusion body by urea concentration and pH gradient ion exchange chromatography
文献类型：期刊论文
| 作者 | Li, M; Su, Z |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2002-07-01 |
| 卷号 | 56期号:1-2页码:33-38 |
| 关键词 | column liquid chromatography ion exchange chromatography size-exclusion chromatography urea concentration refolding of human lysozyme |
| ISSN号 | 0009-5893 |
| 其他题名 | Chromatographia |
| 中文摘要 | A new dual-gradient ion exchange chromatographic method was developed to improve the refolding yield of human lysozyme produced in Escherichia coli as an inclusion body The dissolved and stretched polypeptide chain in a concentrated non-ionic denaturant was adsorbed onto an ion exchange column and induced to refold by gradually decreasing the denaturant concentration and increasing pH in the flowing buffer The dual gradients of denaturant concentration and pH provided a gradual change of the solution environment along the chromatographic column for the protein to refold, resulting in enhanced activity yield and purity. A post-separation was also studied using size-exclusion chromatography to remove protein aggregates and mis-folded proteins after the refolding step. |
| 英文摘要 | A new dual-gradient ion exchange chromatographic method was developed to improve the refolding yield of human lysozyme produced in Escherichia coli as an inclusion body The dissolved and stretched polypeptide chain in a concentrated non-ionic denaturant was adsorbed onto an ion exchange column and induced to refold by gradually decreasing the denaturant concentration and increasing pH in the flowing buffer The dual gradients of denaturant concentration and pH provided a gradual change of the solution environment along the chromatographic column for the protein to refold, resulting in enhanced activity yield and purity. A post-separation was also studied using size-exclusion chromatography to remove protein aggregates and mis-folded proteins after the refolding step. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | SIZE-EXCLUSION CHROMATOGRAPHY ; ESCHERICHIA-COLI ; PROTEINS ; RENATURATION ; BODIES ; YIELD |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000177132900006 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5613]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, M,Su, Z. Refolding human lysozyme produced as an inclusion body by urea concentration and pH gradient ion exchange chromatography[J]. CHROMATOGRAPHIA,2002,56(1-2):33-38. |
| APA | Li, M,&Su, Z.(2002).Refolding human lysozyme produced as an inclusion body by urea concentration and pH gradient ion exchange chromatography.CHROMATOGRAPHIA,56(1-2),33-38. |
| MLA | Li, M,et al."Refolding human lysozyme produced as an inclusion body by urea concentration and pH gradient ion exchange chromatography".CHROMATOGRAPHIA 56.1-2(2002):33-38. |
入库方式： OAI收割
来源：过程工程研究所
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