Refolding of superoxide dismutase by ion-exchange chromatography
文献类型：期刊论文
| 作者 | Li, M; Su, ZG |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2002-06-01 |
| 卷号 | 24期号:11页码:919-923 |
| 关键词 | gradient ion-exchange chromatography inclusion body pH refolding superoxide dismutase urea concentration |
| ISSN号 | 0141-5492 |
| 其他题名 | Biotechnol. Lett. |
| 中文摘要 | A new ion-exchange chromatography process was developed for refolding of iron superoxide dismutase (Fe-SOD) produced in Escherichia coli as an inclusion body. After adsorption on an ion-exchange matrix, the denatured protein was eluted by gradient decrease of urea concentration and pH of the elution buffer. The dual gradient allowed the denatured protein to refold to its correct native conformation with return of biological activity. Compared with the traditional dilution, refolding process, the new process increased the refolding yield five-fold. The process could also be carried out at high protein concentration to decrease the solution volume after refolding. |
| 英文摘要 | A new ion-exchange chromatography process was developed for refolding of iron superoxide dismutase (Fe-SOD) produced in Escherichia coli as an inclusion body. After adsorption on an ion-exchange matrix, the denatured protein was eluted by gradient decrease of urea concentration and pH of the elution buffer. The dual gradient allowed the denatured protein to refold to its correct native conformation with return of biological activity. Compared with the traditional dilution, refolding process, the new process increased the refolding yield five-fold. The process could also be carried out at high protein concentration to decrease the solution volume after refolding. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | ESCHERICHIA-COLI ; LYSOZYME ; RENATURATION ; PROTEINS ; YIELD |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000175752100012 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5614]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, M,Su, ZG. Refolding of superoxide dismutase by ion-exchange chromatography[J]. BIOTECHNOLOGY LETTERS,2002,24(11):919-923. |
| APA | Li, M,&Su, ZG.(2002).Refolding of superoxide dismutase by ion-exchange chromatography.BIOTECHNOLOGY LETTERS,24(11),919-923. |
| MLA | Li, M,et al."Refolding of superoxide dismutase by ion-exchange chromatography".BIOTECHNOLOGY LETTERS 24.11(2002):919-923. |
入库方式： OAI收割
来源：过程工程研究所
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