Dual gradient ion-exchange chromatography improved refolding yield of lysozyme
文献类型：期刊论文
| 作者 | Li, M; Zhang, GF; Su, ZG |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2002-06-14 |
| 卷号 | 959期号:1-2页码:113-120 |
| 关键词 | gradient elution protein folding pH effects lysozyme proteins urea |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | Protein refolding at high concentrations always leads to aggregation, which limits commercial application. An ion-exchange chromatography process with gradient changes in urea concentration and pH was developed to refold denatured lysozyme at high concentration. After adsorption of the denatured protein onto an ion-exchange medium, elution was carried out in combination with a gentle decrease in urea concentration and elevation of pH. Protein would gradually refold along the column with high activity yield. Denatured and reduced lysozyme at 40 mg/ml was loaded into a column filled with SP Sepharose Fast Flow, resulting in 95% activity recovery and 98% mass yield within a short period of time. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | Protein refolding at high concentrations always leads to aggregation, which limits commercial application. An ion-exchange chromatography process with gradient changes in urea concentration and pH was developed to refold denatured lysozyme at high concentration. After adsorption of the denatured protein onto an ion-exchange medium, elution was carried out in combination with a gentle decrease in urea concentration and elevation of pH. Protein would gradually refold along the column with high activity yield. Denatured and reduced lysozyme at 40 mg/ml was loaded into a column filled with SP Sepharose Fast Flow, resulting in 95% activity recovery and 98% mass yield within a short period of time. (C) 2002 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | SIZE-EXCLUSION CHROMATOGRAPHY ; IMMOBILIZED LIPOSOME CHROMATOGRAPHY ; EGG-WHITE LYSOZYME ; ESCHERICHIA-COLI ; INCLUSION-BODIES ; OXIDATIVE RENATURATION ; CARBONIC-ANHYDRASE ; REDUCED LYSOZYME ; PROTEIN ; PURIFICATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000176608400011 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5615]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, M,Zhang, GF,Su, ZG. Dual gradient ion-exchange chromatography improved refolding yield of lysozyme[J]. JOURNAL OF CHROMATOGRAPHY A,2002,959(1-2):113-120. |
| APA | Li, M,Zhang, GF,&Su, ZG.(2002).Dual gradient ion-exchange chromatography improved refolding yield of lysozyme.JOURNAL OF CHROMATOGRAPHY A,959(1-2),113-120. |
| MLA | Li, M,et al."Dual gradient ion-exchange chromatography improved refolding yield of lysozyme".JOURNAL OF CHROMATOGRAPHY A 959.1-2(2002):113-120. |
入库方式： OAI收割
来源：过程工程研究所
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