Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy
文献类型：期刊论文
| 作者 | Shao, MJ; Jiang, L; Cong, W; Ouyang, F |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2002-04-01 |
| 卷号 | 45期号:2页码:149-158 |
| 关键词 | growth of Vero cell microcarrier Cytodex-3 environmental electron scanning microscopy |
| ISSN号 | 1006-9305 |
| 其他题名 | Sci. China Ser. C-Life Sci. |
| 中文摘要 | By using environmental scanning electron microscopy, the morphological changes of Vero cells attached to and grown on the microcarrier Cytodex-3 were observed, and their behavior of adhesion, spreading and proliferation was analyzed. The effect of exogenous fibronectin/laminin on adhesion and spreading of MCC/Vero cell was studied. The images of ESEM showed that expansion of cell growth was directed toward vacancy space. The growth curve and cell concentration change during the whole culture process were obtained from the statistical counting method based on ESEM images and the crystal violet method. The growth rate of Vero cells increases with increasing the concentration of cell inoculation, that is, the specific growth rate increases quickly with increasing the concentration of cell inoculation. When serum concentration in medium #199 ranged from 5% to 10%, experimental results indicated that serum concentration is one of the important factors influencing cell growth, particularly in the cell adhesion and spreading stage. |
| 英文摘要 | By using environmental scanning electron microscopy, the morphological changes of Vero cells attached to and grown on the microcarrier Cytodex-3 were observed, and their behavior of adhesion, spreading and proliferation was analyzed. The effect of exogenous fibronectin/laminin on adhesion and spreading of MCC/Vero cell was studied. The images of ESEM showed that expansion of cell growth was directed toward vacancy space. The growth curve and cell concentration change during the whole culture process were obtained from the statistical counting method based on ESEM images and the crystal violet method. The growth rate of Vero cells increases with increasing the concentration of cell inoculation, that is, the specific growth rate increases quickly with increasing the concentration of cell inoculation. When serum concentration in medium #199 ranged from 5% to 10%, experimental results indicated that serum concentration is one of the important factors influencing cell growth, particularly in the cell adhesion and spreading stage. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000175009100005 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5640]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem Met, Multiphase React Lab, Beijing 100080, Peoples R China 2.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shao, MJ,Jiang, L,Cong, W,et al. Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2002,45(2):149-158. |
| APA | Shao, MJ,Jiang, L,Cong, W,&Ouyang, F.(2002).Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,45(2),149-158. |
| MLA | Shao, MJ,et al."Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 45.2(2002):149-158. |
入库方式： OAI收割
来源：过程工程研究所
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