中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis

文献类型:期刊论文

作者Yang, Q; Hu, XJ; Xu, XM; An, LJ; Yuan, XD; Su, ZG; Janson, JC
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2002
卷号34期号:1页码:6-10
关键词thrombin-like enzyme gussurobin cDNA sequence expression purification
ISSN号0582-9879
其他题名Acta Biochim. Biophys. Sin.
中文摘要Total RNAs were extracted from the venom gland of the snake Gloydius ussuriensis. The cDNA of gussurobin, a thrombin-like enzyme from Gloydius ussuriensis, was cloned and amplified by RT-PCR. Assay of the nucleotide sequence of the cDNA allowed postulation of the complete amino acid sequence for Gloydius ussuriensis, Chinese Viperdae. Its amino acid sequence exhibits significant homology with that of other snake thrombin-like enzymes. The cDNA of gussurobin was inserted into the vector pPIC 9K and expressed successfully in Pichia pastoris, strain GS115. The recombinant gussurobin was separated and purified from 500 ml culture and showed one band on SDS-PAGE.
英文摘要Total RNAs were extracted from the venom gland of the snake Gloydius ussuriensis. The cDNA of gussurobin, a thrombin-like enzyme from Gloydius ussuriensis, was cloned and amplified by RT-PCR. Assay of the nucleotide sequence of the cDNA allowed postulation of the complete amino acid sequence for Gloydius ussuriensis, Chinese Viperdae. Its amino acid sequence exhibits significant homology with that of other snake thrombin-like enzymes. The cDNA of gussurobin was inserted into the vector pPIC 9K and expressed successfully in Pichia pastoris, strain GS115. The recombinant gussurobin was separated and purified from 500 ml culture and showed one band on SDS-PAGE.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]MOLECULAR-CLONING ; SEQUENCE-ANALYSIS ; PICHIA-PASTORIS ; CDNA ; RHODOSTOMA ; BATROXOBIN ; PROTEASES ; ANCROD ; ACID
收录类别SCI
原文出处://WOS:000173426700002
语种英语
WOS记录号WOS:000173426700002
公开日期2013-11-12
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/5664]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Dalian Univ Technol, Bioengn Inst, Dalian 116012, Peoples R China
2.Takara Biotech Dalian Co Ltd, Dalian 116600, Peoples R China
3.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Chem Met, Beijing 100080, Peoples R China
4.Biomed Ctr 577, Biosurface Sci Ctr, S-75123 Uppsala, Sweden
推荐引用方式
GB/T 7714
Yang, Q,Hu, XJ,Xu, XM,et al. Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(1):6-10.
APA Yang, Q.,Hu, XJ.,Xu, XM.,An, LJ.,Yuan, XD.,...&Janson, JC.(2002).Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(1),6-10.
MLA Yang, Q,et al."Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.1(2002):6-10.

入库方式: OAI收割

来源:过程工程研究所

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