Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis
文献类型：期刊论文
| 作者 | Yang, Q; Hu, XJ; Xu, XM; An, LJ; Yuan, XD; Su, ZG; Janson, JC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:1页码:6-10 |
| 关键词 | thrombin-like enzyme gussurobin cDNA sequence expression purification |
| ISSN号 | 0582-9879 |
| 其他题名 | Acta Biochim. Biophys. Sin. |
| 中文摘要 | Total RNAs were extracted from the venom gland of the snake Gloydius ussuriensis. The cDNA of gussurobin, a thrombin-like enzyme from Gloydius ussuriensis, was cloned and amplified by RT-PCR. Assay of the nucleotide sequence of the cDNA allowed postulation of the complete amino acid sequence for Gloydius ussuriensis, Chinese Viperdae. Its amino acid sequence exhibits significant homology with that of other snake thrombin-like enzymes. The cDNA of gussurobin was inserted into the vector pPIC 9K and expressed successfully in Pichia pastoris, strain GS115. The recombinant gussurobin was separated and purified from 500 ml culture and showed one band on SDS-PAGE. |
| 英文摘要 | Total RNAs were extracted from the venom gland of the snake Gloydius ussuriensis. The cDNA of gussurobin, a thrombin-like enzyme from Gloydius ussuriensis, was cloned and amplified by RT-PCR. Assay of the nucleotide sequence of the cDNA allowed postulation of the complete amino acid sequence for Gloydius ussuriensis, Chinese Viperdae. Its amino acid sequence exhibits significant homology with that of other snake thrombin-like enzymes. The cDNA of gussurobin was inserted into the vector pPIC 9K and expressed successfully in Pichia pastoris, strain GS115. The recombinant gussurobin was separated and purified from 500 ml culture and showed one band on SDS-PAGE. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MOLECULAR-CLONING ; SEQUENCE-ANALYSIS ; PICHIA-PASTORIS ; CDNA ; RHODOSTOMA ; BATROXOBIN ; PROTEASES ; ANCROD ; ACID |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000173426700002 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5664]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Dalian Univ Technol, Bioengn Inst, Dalian 116012, Peoples R China 2.Takara Biotech Dalian Co Ltd, Dalian 116600, Peoples R China 3.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Chem Met, Beijing 100080, Peoples R China 4.Biomed Ctr 577, Biosurface Sci Ctr, S-75123 Uppsala, Sweden |
| 推荐引用方式 GB/T 7714 | Yang, Q,Hu, XJ,Xu, XM,et al. Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(1):6-10. |
| APA | Yang, Q.,Hu, XJ.,Xu, XM.,An, LJ.,Yuan, XD.,...&Janson, JC.(2002).Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(1),6-10. |
| MLA | Yang, Q,et al."Cloning, expression and purification of gussurobin, a thrombin-like enzyme from the snake venom of Gloydius ussuriensis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.1(2002):6-10. |
入库方式： OAI收割
来源：过程工程研究所
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