Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis
文献类型：期刊论文
| 作者 | Yang, Q; Xu, XM; Li, M; Yuan, XD; Su, ZG; Janson, JC; An, LJ |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2002 |
| 卷号 | 24期号:2页码:135-138 |
| 关键词 | cDNA sequence defibrase expression separation thrombin-like enzyme |
| ISSN号 | 0141-5492 |
| 其他题名 | Biotechnol. Lett. |
| 中文摘要 | The total RNA was extracted from the venom gland of snake, Gloydius shedaoensis. The defibrase cDNA was amplified by RT-PCR. Assaying the nucleotide sequence of the cDNA allowed the complete amino acid sequence for defibrase of Gloydius shedaoensis to be determined. The defibrase cDNA was inserted into the vector, pPIC 9K, and expressed in Pichia pastoris which then produced 10 mg target protein l(-1). |
| 英文摘要 | The total RNA was extracted from the venom gland of snake, Gloydius shedaoensis. The defibrase cDNA was amplified by RT-PCR. Assaying the nucleotide sequence of the cDNA allowed the complete amino acid sequence for defibrase of Gloydius shedaoensis to be determined. The defibrase cDNA was inserted into the vector, pPIC 9K, and expressed in Pichia pastoris which then produced 10 mg target protein l(-1). |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | THROMBIN-LIKE ENZYME ; SEQUENCE-ANALYSIS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000173417300008 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5666]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Dalian Univ Technol, Bioengn Inst, Dalian 116012, Peoples R China 2.Takara Biotech Dalian Co Ltd, Dalian 116600, Peoples R China 3.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 4.Biomed Ctr, Biosurface Sci Ctr, S-75123 Uppsala, Sweden |
| 推荐引用方式 GB/T 7714 | Yang, Q,Xu, XM,Li, M,et al. Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis[J]. BIOTECHNOLOGY LETTERS,2002,24(2):135-138. |
| APA | Yang, Q.,Xu, XM.,Li, M.,Yuan, XD.,Su, ZG.,...&An, LJ.(2002).Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis.BIOTECHNOLOGY LETTERS,24(2),135-138. |
| MLA | Yang, Q,et al."Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis".BIOTECHNOLOGY LETTERS 24.2(2002):135-138. |
入库方式： OAI收割
来源：过程工程研究所
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