中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis

文献类型:期刊论文

作者Yang, Q; Xu, XM; Li, M; Yuan, XD; Su, ZG; Janson, JC; An, LJ
刊名BIOTECHNOLOGY LETTERS
出版日期2002
卷号24期号:2页码:135-138
关键词cDNA sequence defibrase expression separation thrombin-like enzyme
ISSN号0141-5492
其他题名Biotechnol. Lett.
中文摘要The total RNA was extracted from the venom gland of snake, Gloydius shedaoensis. The defibrase cDNA was amplified by RT-PCR. Assaying the nucleotide sequence of the cDNA allowed the complete amino acid sequence for defibrase of Gloydius shedaoensis to be determined. The defibrase cDNA was inserted into the vector, pPIC 9K, and expressed in Pichia pastoris which then produced 10 mg target protein l(-1).
英文摘要The total RNA was extracted from the venom gland of snake, Gloydius shedaoensis. The defibrase cDNA was amplified by RT-PCR. Assaying the nucleotide sequence of the cDNA allowed the complete amino acid sequence for defibrase of Gloydius shedaoensis to be determined. The defibrase cDNA was inserted into the vector, pPIC 9K, and expressed in Pichia pastoris which then produced 10 mg target protein l(-1).
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]THROMBIN-LIKE ENZYME ; SEQUENCE-ANALYSIS
收录类别SCI
原文出处://WOS:000173417300008
语种英语
WOS记录号WOS:000173417300008
公开日期2013-11-12
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/5666]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Dalian Univ Technol, Bioengn Inst, Dalian 116012, Peoples R China
2.Takara Biotech Dalian Co Ltd, Dalian 116600, Peoples R China
3.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
4.Biomed Ctr, Biosurface Sci Ctr, S-75123 Uppsala, Sweden
推荐引用方式
GB/T 7714
Yang, Q,Xu, XM,Li, M,et al. Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis[J]. BIOTECHNOLOGY LETTERS,2002,24(2):135-138.
APA Yang, Q.,Xu, XM.,Li, M.,Yuan, XD.,Su, ZG.,...&An, LJ.(2002).Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis.BIOTECHNOLOGY LETTERS,24(2),135-138.
MLA Yang, Q,et al."Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis".BIOTECHNOLOGY LETTERS 24.2(2002):135-138.

入库方式: OAI收割

来源:过程工程研究所

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