Selective precipitation of water-soluble proteins using designed polyelectrolyte
文献类型：期刊论文
| 作者 | Yu, J; Liu, HZ; Chen, JY |
| 刊名 | SEPARATION SCIENCE AND TECHNOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 37期号:1页码:217-228 |
| 关键词 | water-soluble protein polyelectrolyte flocculation selective precipitation |
| ISSN号 | 0149-6395 |
| 其他题名 | Sep. Sci. Technol. |
| 中文摘要 | By using lysozyme, hemoglobin (bovine), and pepsin as the model proteins and copolymer F1110 containing reactive functional groups of amido, sulfonate, and carboxylic acid groups as the flocculating agents, selective precipitation of proteins was performed by using two chemical processes. The experiment shows that the surface charge of copolymer F1110 is closely related to the pH value in solution. The flocculation behaviors of proteins with F1110 can be controlled completely by adjusting pH value in solution, which realizes the selective separation of mixed proteins. In addition, the selective precipitation of the proteins is promoted by a "modified flocculation" process, in two steps with a combination of F1110 and polyacrylamide. The characterization of modified flocculation is closely related to the property of the protein. These studies show a great prospect for selectively separating proteins from a mixture solution using designed polyelectrolytes containing reactive functional groups. |
| 英文摘要 | By using lysozyme, hemoglobin (bovine), and pepsin as the model proteins and copolymer F1110 containing reactive functional groups of amido, sulfonate, and carboxylic acid groups as the flocculating agents, selective precipitation of proteins was performed by using two chemical processes. The experiment shows that the surface charge of copolymer F1110 is closely related to the pH value in solution. The flocculation behaviors of proteins with F1110 can be controlled completely by adjusting pH value in solution, which realizes the selective separation of mixed proteins. In addition, the selective precipitation of the proteins is promoted by a "modified flocculation" process, in two steps with a combination of F1110 and polyacrylamide. The characterization of modified flocculation is closely related to the property of the protein. These studies show a great prospect for selectively separating proteins from a mixture solution using designed polyelectrolytes containing reactive functional groups. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Chemistry ; Engineering |
| 关键词[WOS] | POLYMER MOLECULAR-WEIGHT ; FLOCCULATION ; POLYETHYLENEIMINE ; COACERVATION ; ADSORPTION ; SEPARATION ; COPOLYMER ; BEHAVIOR ; RATIO |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000173792700012 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5668]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Chem Met, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, J,Liu, HZ,Chen, JY. Selective precipitation of water-soluble proteins using designed polyelectrolyte[J]. SEPARATION SCIENCE AND TECHNOLOGY,2002,37(1):217-228. |
| APA | Yu, J,Liu, HZ,&Chen, JY.(2002).Selective precipitation of water-soluble proteins using designed polyelectrolyte.SEPARATION SCIENCE AND TECHNOLOGY,37(1),217-228. |
| MLA | Yu, J,et al."Selective precipitation of water-soluble proteins using designed polyelectrolyte".SEPARATION SCIENCE AND TECHNOLOGY 37.1(2002):217-228. |
入库方式： OAI收割
来源：过程工程研究所
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