中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Selective precipitation of water-soluble proteins using designed polyelectrolyte

文献类型:期刊论文

作者Yu, J; Liu, HZ; Chen, JY
刊名SEPARATION SCIENCE AND TECHNOLOGY
出版日期2002
卷号37期号:1页码:217-228
关键词water-soluble protein polyelectrolyte flocculation selective precipitation
ISSN号0149-6395
其他题名Sep. Sci. Technol.
中文摘要By using lysozyme, hemoglobin (bovine), and pepsin as the model proteins and copolymer F1110 containing reactive functional groups of amido, sulfonate, and carboxylic acid groups as the flocculating agents, selective precipitation of proteins was performed by using two chemical processes. The experiment shows that the surface charge of copolymer F1110 is closely related to the pH value in solution. The flocculation behaviors of proteins with F1110 can be controlled completely by adjusting pH value in solution, which realizes the selective separation of mixed proteins. In addition, the selective precipitation of the proteins is promoted by a "modified flocculation" process, in two steps with a combination of F1110 and polyacrylamide. The characterization of modified flocculation is closely related to the property of the protein. These studies show a great prospect for selectively separating proteins from a mixture solution using designed polyelectrolytes containing reactive functional groups.
英文摘要By using lysozyme, hemoglobin (bovine), and pepsin as the model proteins and copolymer F1110 containing reactive functional groups of amido, sulfonate, and carboxylic acid groups as the flocculating agents, selective precipitation of proteins was performed by using two chemical processes. The experiment shows that the surface charge of copolymer F1110 is closely related to the pH value in solution. The flocculation behaviors of proteins with F1110 can be controlled completely by adjusting pH value in solution, which realizes the selective separation of mixed proteins. In addition, the selective precipitation of the proteins is promoted by a "modified flocculation" process, in two steps with a combination of F1110 and polyacrylamide. The characterization of modified flocculation is closely related to the property of the protein. These studies show a great prospect for selectively separating proteins from a mixture solution using designed polyelectrolytes containing reactive functional groups.
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Multidisciplinary ; Engineering, Chemical
研究领域[WOS]Chemistry ; Engineering
关键词[WOS]POLYMER MOLECULAR-WEIGHT ; FLOCCULATION ; POLYETHYLENEIMINE ; COACERVATION ; ADSORPTION ; SEPARATION ; COPOLYMER ; BEHAVIOR ; RATIO
收录类别SCI
原文出处://WOS:000173792700012
语种英语
WOS记录号WOS:000173792700012
公开日期2013-11-12
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/5668]  
专题过程工程研究所_研究所(批量导入)
作者单位Chinese Acad Sci, Inst Chem Met, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China
推荐引用方式
GB/T 7714
Yu, J,Liu, HZ,Chen, JY. Selective precipitation of water-soluble proteins using designed polyelectrolyte[J]. SEPARATION SCIENCE AND TECHNOLOGY,2002,37(1):217-228.
APA Yu, J,Liu, HZ,&Chen, JY.(2002).Selective precipitation of water-soluble proteins using designed polyelectrolyte.SEPARATION SCIENCE AND TECHNOLOGY,37(1),217-228.
MLA Yu, J,et al."Selective precipitation of water-soluble proteins using designed polyelectrolyte".SEPARATION SCIENCE AND TECHNOLOGY 37.1(2002):217-228.

入库方式: OAI收割

来源:过程工程研究所

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