Mechanisms of protein fouling in microfiltration. I. Determination of proteins fouled on microfiltration membranes
文献类型：期刊论文
| 作者 | Zhang, JD; Cai, ZL; Cong, W; Su, ZG; Fan, OY |
| 刊名 | SEPARATION SCIENCE AND TECHNOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 37期号:13页码:3025-3038 |
| 关键词 | quantification protein fouling microfiltration |
| ISSN号 | 0149-6395 |
| 其他题名 | Sep. Sci. Technol. |
| 中文摘要 | Protein fouling is one of the critical factors governing the effectiveness of many microfiltration processes. Although mechanisms have been proposed, direct correlation between protein amount fouled on membranes and filtration behavior is required. We developed a simple method to measure proteins on microfiltration membranes. The protein-fouled membranes were stained by amido black 10B first, then destained to remove excess dye not bound to protein. Finally, the dye associated with the protein on the membranes was eluted with 0.1 N NaOH. Three membranes (mixed esters of cellulose nitrate and acetate membrane, Durapore GVWP membrane, and. nuclear-pore membrane) were examined by blotting, adsorbing, and depositing bovine serum albumin on them. The absorbency of the eluted solutions was measured at 620 nm. The absorbency of the eluted solutions was independent of staining time and eluting time, but decreased with prolonged destaining time. The results showed a good linear relationship between the absorbency and amount of protein on membranes in all conditions examined. Three other proteins were also examined and the results showed that different proteins have different slopes. These results indicate that the method could be used to quantify proteins fouled on membrane. |
| 英文摘要 | Protein fouling is one of the critical factors governing the effectiveness of many microfiltration processes. Although mechanisms have been proposed, direct correlation between protein amount fouled on membranes and filtration behavior is required. We developed a simple method to measure proteins on microfiltration membranes. The protein-fouled membranes were stained by amido black 10B first, then destained to remove excess dye not bound to protein. Finally, the dye associated with the protein on the membranes was eluted with 0.1 N NaOH. Three membranes (mixed esters of cellulose nitrate and acetate membrane, Durapore GVWP membrane, and. nuclear-pore membrane) were examined by blotting, adsorbing, and depositing bovine serum albumin on them. The absorbency of the eluted solutions was measured at 620 nm. The absorbency of the eluted solutions was independent of staining time and eluting time, but decreased with prolonged destaining time. The results showed a good linear relationship between the absorbency and amount of protein on membranes in all conditions examined. Three other proteins were also examined and the results showed that different proteins have different slopes. These results indicate that the method could be used to quantify proteins fouled on membrane. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Chemistry ; Engineering |
| 关键词[WOS] | ULTRAFILTRATION MEMBRANES ; ADSORPTION ; BEHAVIOR ; BSA |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000178523600004 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5679]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn China, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, JD,Cai, ZL,Cong, W,et al. Mechanisms of protein fouling in microfiltration. I. Determination of proteins fouled on microfiltration membranes[J]. SEPARATION SCIENCE AND TECHNOLOGY,2002,37(13):3025-3038. |
| APA | Zhang, JD,Cai, ZL,Cong, W,Su, ZG,&Fan, OY.(2002).Mechanisms of protein fouling in microfiltration. I. Determination of proteins fouled on microfiltration membranes.SEPARATION SCIENCE AND TECHNOLOGY,37(13),3025-3038. |
| MLA | Zhang, JD,et al."Mechanisms of protein fouling in microfiltration. I. Determination of proteins fouled on microfiltration membranes".SEPARATION SCIENCE AND TECHNOLOGY 37.13(2002):3025-3038. |
入库方式： OAI收割
来源：过程工程研究所
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