Forward and backward extraction of BSA using mixed reverse micellar system of CTAB and alkyl halides
文献类型：期刊论文
| 作者 | Zhang, W; Liu, HZ; Chen, JY |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2002-10-01 |
| 卷号 | 12期号:1页码:1-5 |
| 关键词 | reverse micelles backward extraction alkyl halide BSA CTAB |
| ISSN号 | 1369-703X |
| 其他题名 | Biochem. Eng. J. |
| 中文摘要 | The forward and backward extraction of bovine serum albumin (BSA) has been studied using mixed reverse micellar systems of cetyl trimethyl ammoniumbromide (CTAB) and alkyl halides (including 1-chlorobutane (R4Cl), 1-bromooctane (R8Br) and 1-iodobutane (R4I)). The addition of alkyl halide R8Br to 50 mmolA CTAB/20% (v/v) hexanol/petroleurn ether reverse micellar system could improve the BSA transfer from the aqueous phase to reverse micellar phase, while the addition of R4Cl or R4I had almost no effect on the BSA transfer. The mixed reverse micelles formed with CTAB and alkyl halides exhibit excellent backward extraction behavior for BSA. The mixed reverse micelles formed with CTAB and R4I can realize the recovery of BSA effectively in a wide range of pH up to or higher than the isoclectric point of BSA. The mixed reverse trucelles formed with CTAB and R4Cl, R8Br or R4I can obviously enhance the BSA backward transfer at low ionic strength with addition of KBr or KCl as electrolyte. The mixed reverse micellar system indicates that it requires less time to reach the mass transfer equilibrium in comparison with the reverse micellar system with CTAB only. The mechanism of backward extraction proposed that with the addition of alkyl halides to CTAB reverse micelles, the hydrophobic interaction between the reverse micelles decreased. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | The forward and backward extraction of bovine serum albumin (BSA) has been studied using mixed reverse micellar systems of cetyl trimethyl ammoniumbromide (CTAB) and alkyl halides (including 1-chlorobutane (R4Cl), 1-bromooctane (R8Br) and 1-iodobutane (R4I)). The addition of alkyl halide R8Br to 50 mmolA CTAB/20% (v/v) hexanol/petroleurn ether reverse micellar system could improve the BSA transfer from the aqueous phase to reverse micellar phase, while the addition of R4Cl or R4I had almost no effect on the BSA transfer. The mixed reverse micelles formed with CTAB and alkyl halides exhibit excellent backward extraction behavior for BSA. The mixed reverse micelles formed with CTAB and R4I can realize the recovery of BSA effectively in a wide range of pH up to or higher than the isoclectric point of BSA. The mixed reverse trucelles formed with CTAB and R4Cl, R8Br or R4I can obviously enhance the BSA backward transfer at low ionic strength with addition of KBr or KCl as electrolyte. The mixed reverse micellar system indicates that it requires less time to reach the mass transfer equilibrium in comparison with the reverse micellar system with CTAB only. The mechanism of backward extraction proposed that with the addition of alkyl halides to CTAB reverse micelles, the hydrophobic interaction between the reverse micelles decreased. (C) 2002 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | PHOSPHORIC-ACID ; AMINO-ACIDS ; PROTEINS ; RECOVERY ; SULFOSUCCINATE ; WATER ; DYE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000178450600001 |
| 公开日期 | 2013-11-12 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5684]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, W,Liu, HZ,Chen, JY. Forward and backward extraction of BSA using mixed reverse micellar system of CTAB and alkyl halides[J]. BIOCHEMICAL ENGINEERING JOURNAL,2002,12(1):1-5. |
| APA | Zhang, W,Liu, HZ,&Chen, JY.(2002).Forward and backward extraction of BSA using mixed reverse micellar system of CTAB and alkyl halides.BIOCHEMICAL ENGINEERING JOURNAL,12(1),1-5. |
| MLA | Zhang, W,et al."Forward and backward extraction of BSA using mixed reverse micellar system of CTAB and alkyl halides".BIOCHEMICAL ENGINEERING JOURNAL 12.1(2002):1-5. |
入库方式： OAI收割
来源：过程工程研究所
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