MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production
文献类型：期刊论文
| 作者 | Li, Dongsheng1; Dong, Bilin1; Tong, Zhongsheng1; Wang, Qinning2; Liu, Weihuang3; Wang, Yan4; Liu, Wei4; Chen, Jinbo1; Xu, Li1; Chen, Liuqing1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012-12-11 |
| 卷号 | 7期号:12页码:e50589 |
| 关键词 | MANNAN-BINDING LECTIN FUNGAL-INFECTIONS DECTIN-1 RECOGNITION ACTIVATION RECEPTOR MANNOSE YEAST CELLS |
| ISSN号 | 1932-6203 |
| 通讯作者 | Chen, LQ (reprint author), 1 Hosp Wuhan, Dept Dermatol, Ctr Infect Skin Dis, Wuhan, Peoples R China. |
| 中文摘要 | Mannan-binding lectin (MBL), a lectin homologous to C1q, greatly facilitates C3/C4-mediated opsonophagocytosis of Candida albicans (C. albicans) by human neutrophils, and has the capacity to bind to CR1 (CD35) expressed on circulating neutrophils. The intracellular pool of neutrophil Dectin-1 plays a critical role in stimulating the reactive oxygen species (ROS) generation through recognition of beta-1,3-glucan component of phagocytized zymosan or yeasts. However, little is known about whether MBL can mediate the opsonophagocytosis of Candida albicans by neutrophils independent of complement activation, and whether MBL-mediated opsonophagocytosis influence the intracellular expression of Dectin-1 and ROS production. Here we showed that the inhibited phagocytic efficiency of neutrophils as a result of blockage of Dectin-1 was compensated by exogenous MBL alone in a dose-dependent manner. Furthermore, the expressions of Dectin-1 at mRNA and intracellular protein levels were significantly up-regulated in neutrophils stimulated by MBL-pre-incubated C. albicans, while the expression of surface Dectin-1 remained almost unchanged. Nevertheless, the stimulated ROS production in neutrophils was partly and irreversibly inhibited by blockage of Dectin-1 in the presence of exogenous MBL. Confocal microscopy examination showed that intracellular Dectin-1 was recruited and co-distributed with ROS on the surface of some phagocytized yeasts. The beta-1,3-glucanase digestion test further suggested that the specific recognition and binding site of human Dectin-1 is just the beta-1,3-glucan moiety on the cell wall of C. albicans. These data demonstrate that MBL has an ability to mediate the opsonophagocytosis of Candida albicans by human neutrophils independent of complement activation, which is coupled with intracellular Dectin-1-triggered ROS production. |
| 英文摘要 | Mannan-binding lectin (MBL), a lectin homologous to C1q, greatly facilitates C3/C4-mediated opsonophagocytosis of Candida albicans (C. albicans) by human neutrophils, and has the capacity to bind to CR1 (CD35) expressed on circulating neutrophils. The intracellular pool of neutrophil Dectin-1 plays a critical role in stimulating the reactive oxygen species (ROS) generation through recognition of beta-1,3-glucan component of phagocytized zymosan or yeasts. However, little is known about whether MBL can mediate the opsonophagocytosis of Candida albicans by neutrophils independent of complement activation, and whether MBL-mediated opsonophagocytosis influence the intracellular expression of Dectin-1 and ROS production. Here we showed that the inhibited phagocytic efficiency of neutrophils as a result of blockage of Dectin-1 was compensated by exogenous MBL alone in a dose-dependent manner. Furthermore, the expressions of Dectin-1 at mRNA and intracellular protein levels were significantly up-regulated in neutrophils stimulated by MBL-pre-incubated C. albicans, while the expression of surface Dectin-1 remained almost unchanged. Nevertheless, the stimulated ROS production in neutrophils was partly and irreversibly inhibited by blockage of Dectin-1 in the presence of exogenous MBL. Confocal microscopy examination showed that intracellular Dectin-1 was recruited and co-distributed with ROS on the surface of some phagocytized yeasts. The beta-1,3-glucanase digestion test further suggested that the specific recognition and binding site of human Dectin-1 is just the beta-1,3-glucan moiety on the cell wall of C. albicans. These data demonstrate that MBL has an ability to mediate the opsonophagocytosis of Candida albicans by human neutrophils independent of complement activation, which is coupled with intracellular Dectin-1-triggered ROS production. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MANNAN-BINDING LECTIN ; FUNGAL-INFECTIONS ; DECTIN-1 ; RECOGNITION ; ACTIVATION ; RECEPTOR ; MANNOSE ; YEAST ; CELLS |
| 收录类别 | SCI |
| 资助信息 | Wuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25] |
| 语种 | 英语 |
| WOS记录号 | WOS:000312290800020 |
| 公开日期 | 2013-11-14 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19362]  |
| 专题 | 水生生物研究所_分析测试中心_期刊论文 |
| 作者单位 | 1.1 Hosp Wuhan, Dept Dermatol, Ctr Infect Skin Dis, Wuhan, Peoples R China 2.Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Westmead, NSW 2145, Australia 3.Wuhan Univ, Med Res Ctr, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Dongsheng,Dong, Bilin,Tong, Zhongsheng,et al. MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production[J]. PLOS ONE,2012,7(12):e50589. |
| APA | Li, Dongsheng.,Dong, Bilin.,Tong, Zhongsheng.,Wang, Qinning.,Liu, Weihuang.,...&Duan, Yiqun.(2012).MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production.PLOS ONE,7(12),e50589. |
| MLA | Li, Dongsheng,et al."MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production".PLOS ONE 7.12(2012):e50589. |
入库方式： OAI收割
来源：水生生物研究所
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