High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli
文献类型：期刊论文
| 作者 | Wang, Dan1,2; Li, Qiang1,2; Mao, Yu3; Xing, Jianmin1; Su, Zhiguo1 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2010-08-01 |
| 卷号 | 87期号:6页码:2025-2035 |
| 关键词 | Glucose phosphotransferase (ptsG) Escherichia coli Succinic acid Lactose induction Phosphoenolpyruvate carboxylase (ppc) |
| ISSN号 | 0175-7598 |
| 通讯作者 | Xing, JM |
| 英文摘要 | Escherichia coli strains with foreign genes under the isopropyl-beta-d-thiogalactopyranoside-inducible promoters such as lac, tac, and trc were engineered and considered as the promising succinic acid-producing bacteria in many reports. The promoters mentioned above could also be induced by lactose, which had not been attempted for succinic acid production before. Here, the efficient utilization of lactose as inducer was demonstrated in cultures of the ptsG, ldhA, and pflB mutant strain DC1515 with ppc overexpression. A fermentative process for succinic acid production at high level by this strain was developed. In flask anaerobic culture, 14.86 g l(-1) succinic acid was produced from 15 g l(-1) glucose with a yield of 1.51 mol mol(-1) glucose. In two-stage culture carried out in a 3-l bioreactor, the overall yield and concentration of succinic acid reached to 1.67 mol mol(-1) glucose and 99.7 g l(-1), respectively, with a productivity of 1.7 g l(-1) h(-1) in the anaerobic stage. The efficient utilization of lactose as inducer made recombinant E. coli a more capable strain for succinic acid production at large scale. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RECOMBINANT PROTEIN-PRODUCTION ; FED-BATCH CULTURE ; PYRUVATE-CARBOXYLASE ; LAC PROMOTER ; STRAIN ; OVEREXPRESSION ; GENE ; FERMENTATION ; METABOLISM ; GLUCOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280373100005 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5835]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.China Univ Min & Technol Beijing, Sch Chem & Environm Engn, Dept Bioengn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dan,Li, Qiang,Mao, Yu,et al. High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,87(6):2025-2035. |
| APA | Wang, Dan,Li, Qiang,Mao, Yu,Xing, Jianmin,&Su, Zhiguo.(2010).High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,87(6),2025-2035. |
| MLA | Wang, Dan,et al."High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 87.6(2010):2025-2035. |
入库方式： OAI收割
来源：过程工程研究所
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