Biochemical characterization of a maize stover beta-exoglucanase and its use in lignocellulose conversion
文献类型：期刊论文
| 作者 | Han, Yejun; Chen, Hongzhang
|
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2010-08-01 |
| 卷号 | 101期号:15页码:6111-6117 |
| 关键词 | beta-Exoglucanase Maize stover Synergism Simultaneous saccharification and fermentation (SSF) |
| ISSN号 | 0960-8524 |
| 通讯作者 | Chen, HZ |
| 英文摘要 | Plant is one of the important resources for glycosyl hydrolase production. A beta-exoglucanase with molecular weight of 63.1 kDa was purified from fresh maize stover and subjected to enzymatic characterization. The optimal temperature and pH of the beta-exoglucanase was 40 degrees C and 6.0, respectively. The beta-exoglucanase was active against p-nitrophenyl-cellobiose (p-NPC), laminarin, cellotriose, cellotetraose, cellopentaose, Avicel, filter paper, and cotton cellulose. The analysis of hydrolytic mode suggested that the beta-exoglucanase removed cellobiose from the ends of beta-glucan. Kinetic parameters of the beta-exoglucanase for laminarin and p-NPC were determined. The effects of metal ions and chemical reagents on the beta-exoglucanase activity were also studied. The biochemical characterization of the beta-exoglucanase makes it an appealing cellulase additive in converting lignocelluloses to ethanol through simultaneous saccharification and fermentation. The synergism of the beta-exoglucanase or crude cell wall proteins of fresh maize stover with Trichoderma reesei cellulase was observed in ethanol production from lignocellulose. (C) 2010 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | COLEOPTILE CELL-WALLS ; SIMULTANEOUS SACCHARIFICATION ; MIXED LINKAGE ; CORN STOVER ; HYDROLASES ; ENDOGLUCANASES ; FERMENTATION ; PURIFICATION ; GLUCOSIDASE ; DEGRADATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278035900051 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5837]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yejun,Chen, Hongzhang. Biochemical characterization of a maize stover beta-exoglucanase and its use in lignocellulose conversion[J]. BIORESOURCE TECHNOLOGY,2010,101(15):6111-6117. |
| APA | Han, Yejun,&Chen, Hongzhang.(2010).Biochemical characterization of a maize stover beta-exoglucanase and its use in lignocellulose conversion.BIORESOURCE TECHNOLOGY,101(15),6111-6117. |
| MLA | Han, Yejun,et al."Biochemical characterization of a maize stover beta-exoglucanase and its use in lignocellulose conversion".BIORESOURCE TECHNOLOGY 101.15(2010):6111-6117. |
入库方式： OAI收割
来源：过程工程研究所
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