Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography
文献类型：期刊论文
| 作者 | Liu, Dan2; Su, Zhiguo1; Wang, Changhai2; Gu, Ming1; Xing, Siliang3 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2010-08-01 |
| 卷号 | 33期号:15页码:2266-2271 |
| 关键词 | Corilagin Gallic acid Geraniin Normal-phase high-speed counter-current chromatography Reversed-phase high-speed counter-current chromatography |
| ISSN号 | 1615-9306 |
| 通讯作者 | Gu, M |
| 英文摘要 | Three hydrolyzable tannins, geraniin, corilagin and gallic acid, main active components of Geranium wilfordii Maxim, have been separated and purified in one-step by both reversed-phase and normal-phase high-speed counter-current chromatography. Gallic acid, corilagin and geraniin were purified from 70% aqueous acetone extract of G. wilfordii Maxim with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:10:0.2:0.2:20) by reversed-phase high-speed counter-current chromatography at purities of 94.2, 91.0 and 91.3%, at yields of 89.3, 82.9 and 91.7%, respectively. Gallic acid, corilagin and geraniin were purified with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (0.2:10:2:1:5) by normal-phase high-speed counter-current chromatography at purities of 85.9, 92.2 and 87.6%, at yields of 87.4, 94.6 and 94.3%, respectively. It was successful for both reversed-phase and normal-phase high-speed counter-current chromatography to separate high-polarity of low-molecular-weight substances. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | PHYLLANTHUS-URINARIA ; PARTITION CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; GALLIC ACID ; BUNGE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281055900006 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6110]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian, Peoples R China 3.Beijing Kangmingyousheng Biochem Technol Co Ltd, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Dan,Su, Zhiguo,Wang, Changhai,et al. Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(15):2266-2271. |
| APA | Liu, Dan,Su, Zhiguo,Wang, Changhai,Gu, Ming,&Xing, Siliang.(2010).Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,33(15),2266-2271. |
| MLA | Liu, Dan,et al."Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase highspeed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 33.15(2010):2266-2271. |
入库方式： OAI收割
来源：过程工程研究所
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