A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall
文献类型：期刊论文
| 作者 | Han, Yejun; Chen, Hongzhang
|
| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 63期号:3-4页码:135-140 |
| 关键词 | beta-Xylosidase Plant cell wall proteins Hemicellulose Enzymatic hydrolysis |
| ISSN号 | 1381-1177 |
| 通讯作者 | Chen, HZ |
| 英文摘要 | To study the potential of plant glycoside hydrolase for hemicelluloses hydrolysis, a beta-xylosidase with molecular weight of 68.5 kDa was purified from the maize during senescent stage. The optimal conditions for the beta-xylosidase were 37 degrees C and pH 4.5. In absence of substrate, the beta-xylosidase was comparatively stable at 37 degrees C and pH 4.5-5.5. At the optimum condition, the K(m) and k(cat) values of the beta-xylosidase against p-nitrophenyl-xyloside were 2.5 mM and 6.5 s(-1), respectively. The enzyme activity was promoted by LiCl, CaCl(2), MnCl(2), MgCl(2), KCl. and NaCl, however severely inhibited by CuCl(2), ZnCl(2), AgNO(3), HgCl(2), and NiCl(2). The purified beta-xylosidase was active against xylobiose, xylotriose, xylotetraose, and xylopentaose. In hydrolysis of corn stover hemicellulose, the xylose production increased by 94.9% and 140% when Trichoderma reesei hemicellulase supplemented with purified beta-xylosidase and crude cell wall proteins of corn stover, respectively. The biochemical characterization of the maize beta-xylosidase makes it a promising candidate enzyme additive for hemicelluloses hydrolysis. (C) 2010 Elsevier By. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Physical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | MOLECULAR-BIOLOGY ; PROTEINS ; EXTRACTION ; XYLANASE ; TRANSXYLOSYLATION ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276116900005 |
| 公开日期 | 2013-11-19 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6158]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yejun,Chen, Hongzhang. A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2010,63(3-4):135-140. |
| APA | Han, Yejun,&Chen, Hongzhang.(2010).A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,63(3-4),135-140. |
| MLA | Han, Yejun,et al."A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 63.3-4(2010):135-140. |
入库方式： OAI收割
来源：过程工程研究所
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