Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs
文献类型：期刊论文
| 作者 | Li, C; Bai, JH; Li, W; Cai, ZL; Ouyang, F |
| 刊名 | BIOTECHNOLOGY PROGRESS
 |
| 出版日期 | 2001-03-01 |
| 卷号 | 17期号:2页码:366-368 |
| 关键词 | lactic-acid bacteria response-surface methodology subsp lactis nisin purification |
| ISSN号 | 8756-7938 |
| 其他题名 | Biotechnol. Prog. |
| 中文摘要 | The bacteriocin nisin was extracted in PEG/salt aqueous two-phase systems (ATPS) using the property that the systems can extract hydrophobic proteins. The concentrations of the phase-forming components, PEG 4000 and Na2SO4, were optimized for nisin recovery by means of statistical experimental designs, and it was found that they strongly influenced nisin recovery. The optimal composition of ATPS was found to be 15.99% (w/w) PEG 4000 and 15.85% (w/w) Na2SO4 (pH 2), and the optimal ATPS allowed an 11.60% increase of nisin recovery compared to the standard method of nisin assay. |
| 英文摘要 | The bacteriocin nisin was extracted in PEG/salt aqueous two-phase systems (ATPS) using the property that the systems can extract hydrophobic proteins. The concentrations of the phase-forming components, PEG 4000 and Na2SO4, were optimized for nisin recovery by means of statistical experimental designs, and it was found that they strongly influenced nisin recovery. The optimal composition of ATPS was found to be 15.99% (w/w) PEG 4000 and 15.85% (w/w) Na2SO4 (pH 2), and the optimal ATPS allowed an 11.60% increase of nisin recovery compared to the standard method of nisin assay. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 关键词[WOS] | LACTIC-ACID BACTERIA ; RESPONSE-SURFACE METHODOLOGY ; SUBSP LACTIS ; NISIN ; PURIFICATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000168044700022 |
| 公开日期 | 2013-11-14 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5772]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Chem Met, Beijing 100080, Peoples R China 2.Hubei Univ, Sch Life Sci, Wuhan 430062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, C,Bai, JH,Li, W,et al. Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs[J]. BIOTECHNOLOGY PROGRESS,2001,17(2):366-368. |
| APA | Li, C,Bai, JH,Li, W,Cai, ZL,&Ouyang, F.(2001).Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs.BIOTECHNOLOGY PROGRESS,17(2),366-368. |
| MLA | Li, C,et al."Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs".BIOTECHNOLOGY PROGRESS 17.2(2001):366-368. |
入库方式： OAI收割
来源：过程工程研究所
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