Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa
文献类型：期刊论文
| 作者 | Zhao, DX; Xing, JM; Li, MY; Lu, DP; Zhao, Q |
| 刊名 | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| 出版日期 | 2001-12-01 |
| 卷号 | 67期号:3页码:227-234 |
| 关键词 | high performance liquid chromatography jaceosidin plant cell culture Saussurea medusa |
| ISSN号 | 0167-6857 |
| 其他题名 | Plant Cell Tissue Organ Cult. |
| 中文摘要 | Calli cultures derived from the leaves of Saussurea medusa were selected on the basis of colour into three callus, A, B and C, which suggested different levels of metabolite accumulation. An improved reversed phase high performance liquid chromatographic method provided selective determination of the jaceosidin content of these samples. The jaceosidin concentration in callus B was higher than that of the callus A and C. By using 12-day old culture and 9-day old inoculum, jaceosidin yield of 72.91 mg l(-1)was obtained from cell line B in cell suspension cultures. The influence of some factors affecting jaceosidin formation, i.e. temperature, light, inoculum size, type of media, phytohormones, nitrogen and carbon source etc. were also examined. Light irradiation and combination of 3% (w/v) sucrose with 1% glucose brought about a marked increase of jaceosidin production. The effect of blue light on jaceosidin was markedly superior to other kinds of monochromatic light (red and far-red) or white light. Analysis of growth and jaceosidin content of callus cultures and cell suspension cultures demonstrated that the production of jaceosidin was growth-dependent in both cell solid culture and cell suspension culture. |
| 英文摘要 | Calli cultures derived from the leaves of Saussurea medusa were selected on the basis of colour into three callus, A, B and C, which suggested different levels of metabolite accumulation. An improved reversed phase high performance liquid chromatographic method provided selective determination of the jaceosidin content of these samples. The jaceosidin concentration in callus B was higher than that of the callus A and C. By using 12-day old culture and 9-day old inoculum, jaceosidin yield of 72.91 mg l(-1)was obtained from cell line B in cell suspension cultures. The influence of some factors affecting jaceosidin formation, i.e. temperature, light, inoculum size, type of media, phytohormones, nitrogen and carbon source etc. were also examined. Light irradiation and combination of 3% (w/v) sucrose with 1% glucose brought about a marked increase of jaceosidin production. The effect of blue light on jaceosidin was markedly superior to other kinds of monochromatic light (red and far-red) or white light. Analysis of growth and jaceosidin content of callus cultures and cell suspension cultures demonstrated that the production of jaceosidin was growth-dependent in both cell solid culture and cell suspension culture. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Plant Sciences |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Plant Sciences |
| 关键词[WOS] | FLAVONOIDS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000172074000003 |
| 公开日期 | 2013-11-14 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5773]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Chem Met, Beijing 100080, Peoples R China 3.China Agr Univ, Beijing 100094, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, DX,Xing, JM,Li, MY,et al. Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2001,67(3):227-234. |
| APA | Zhao, DX,Xing, JM,Li, MY,Lu, DP,&Zhao, Q.(2001).Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa.PLANT CELL TISSUE AND ORGAN CULTURE,67(3),227-234. |
| MLA | Zhao, DX,et al."Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa".PLANT CELL TISSUE AND ORGAN CULTURE 67.3(2001):227-234. |
入库方式： OAI收割
来源：过程工程研究所
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