Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos
文献类型：期刊论文
| 作者 | Tang, W; Guo, ZC; Ouyang, F |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
 |
| 出版日期 | 2001-09-01 |
| 卷号 | 37期号:5页码:558-563 |
| 关键词 | Pinus taeda L. embryogenic cultures somatic embryogenesis plant regeneration |
| ISSN号 | 1054-5476 |
| 其他题名 | In Vitro Cell. Dev. Biol.-Plant |
| 中文摘要 | Mature zygotic embryos of eight (open-pollinated) families of loblolly pine (Pinus taeda L.) were cultured on eight different basal salt formulations, each supplemented with 36.2 muM 2,4-dichlorophenoxyacetic acid, 17.8 muM 6-benzyladenine, 18.6 muM kinetin, 500 mg l(-1) casein hydrolysate, and 500 mg l(-1) L-glutamine for 9 wk; embryogenic tissue was formed on cotyledons, hypocotyls, and radicles of mature zygotic embryos. Callus was subcultured on the callus, proliferation, medium, the same as the induction medium, bat with one-fifth concentration of auxin and cytokinin for 9 wk. On this medium a white to translucent, glossy, mucilaginous embryogenic callus containing embryogenic suspensor masses (ESMs) was obtained. The highest frequency of explants forming embryogenic tissue, 17%, occurred on a modified Murashige and Skoog salts basal medium containing the concentration of KNO3, Ca(NO3)(2)-4H(2)O, NH4NO3, KCI, ZnSO4-7H(2)O(2), and MnSO4-H2O, 720, 1900, 00 250, 25.8, and 25.35 mg l(-1), respectively. Embryogenic suspension cultures were established by culturing embryogenic callus in liquid callus proliferation medium. Liquid cultures containing ESMs were transferred to medium containing abscisic acid, polyethylene glycols, and activated charcoal for stimulating ther production of cotyledonary somatic embryos. Mature somatic embryos germinated for 4-12 wk on medium containing indole-butyric acid, gibberellic acid, 6-benzyladenine, activated charcoal, and reduced sucrose concentration (15 g l(-1)). Two hundred and ninety-one regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1:1:1) mixture, then the plants were transplanted to soil in the earth, and 73 plantlets survived in the field. |
| 英文摘要 | Mature zygotic embryos of eight (open-pollinated) families of loblolly pine (Pinus taeda L.) were cultured on eight different basal salt formulations, each supplemented with 36.2 muM 2,4-dichlorophenoxyacetic acid, 17.8 muM 6-benzyladenine, 18.6 muM kinetin, 500 mg l(-1) casein hydrolysate, and 500 mg l(-1) L-glutamine for 9 wk; embryogenic tissue was formed on cotyledons, hypocotyls, and radicles of mature zygotic embryos. Callus was subcultured on the callus, proliferation, medium, the same as the induction medium, bat with one-fifth concentration of auxin and cytokinin for 9 wk. On this medium a white to translucent, glossy, mucilaginous embryogenic callus containing embryogenic suspensor masses (ESMs) was obtained. The highest frequency of explants forming embryogenic tissue, 17%, occurred on a modified Murashige and Skoog salts basal medium containing the concentration of KNO3, Ca(NO3)(2)-4H(2)O, NH4NO3, KCI, ZnSO4-7H(2)O(2), and MnSO4-H2O, 720, 1900, 00 250, 25.8, and 25.35 mg l(-1), respectively. Embryogenic suspension cultures were established by culturing embryogenic callus in liquid callus proliferation medium. Liquid cultures containing ESMs were transferred to medium containing abscisic acid, polyethylene glycols, and activated charcoal for stimulating ther production of cotyledonary somatic embryos. Mature somatic embryos germinated for 4-12 wk on medium containing indole-butyric acid, gibberellic acid, 6-benzyladenine, activated charcoal, and reduced sucrose concentration (15 g l(-1)). Two hundred and ninety-one regenerated plantlets were transferred to a perlite:peatmoss:vermiculite (1:1:1) mixture, then the plants were transplanted to soil in the earth, and 73 plantlets survived in the field. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 研究领域[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
| 关键词[WOS] | SOMATIC EMBRYOGENESIS ; PICEA-GLAUCA ; STORED SEEDS ; WHITE SPRUCE ; CALLUS ; POLYEMBRYOGENESIS ; INDUCTION ; CONIFERS ; MARIANA ; BLACK |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000172369800009 |
| 公开日期 | 2013-11-14 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5777]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Lab Plant Cell Engn, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Chem Met, Biochem Engn Lab, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, W,Guo, ZC,Ouyang, F. Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2001,37(5):558-563. |
| APA | Tang, W,Guo, ZC,&Ouyang, F.(2001).Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,37(5),558-563. |
| MLA | Tang, W,et al."Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 37.5(2001):558-563. |
入库方式： OAI收割
来源：过程工程研究所
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