Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase
文献类型：期刊论文
| 作者 | Liu, JG; Cong, W; Wang, S; Ouyang, F |
| 刊名 | REACTIVE & FUNCTIONAL POLYMERS
 |
| 出版日期 | 2001-05-02 |
| 卷号 | 48期号:1-3页码:75-84 |
| 关键词 | poly(vinyl acetate-co-diviyl benzene) stability immobilization penicillin acylase kinetics |
| ISSN号 | 1381-5148 |
| 其他题名 | React. Funct. Polym. |
| 中文摘要 | Poly(vinyl acetate-co-diviyl benzene) beads were synthesized via a novel polymerization method. The amount of hydroxide radical could reach 11.5 mmol g(-1) (wet) after the beads were catalyzed by NaOH in methanol at 18-20 degreesC for 24 h. The activators such as SESA, glutaraldehyde and p-benzoquinone were used to immobilize penicillin acylase. The penicillin acylase immobilized with SESA exhibited better stability than that with other activators. The successively immobilization could increase the activity of immobilized penicillin acylase about two times and the activity yield 10%, respectively, At the optimal conditions of immobilization, the activity of immobilized penicillin acylase amounted to 812-1200 unit per gram (wet). The kinetics of immobilized penicillin acylase was also studied. The reaction constants, K,, K-iPAA K-iAPA and K-s for immobilized penicillin acylase were equal to 0.033, 0.013, 0.009 and 0.081 mmol/ml, respectively. (C) 2001 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | Poly(vinyl acetate-co-diviyl benzene) beads were synthesized via a novel polymerization method. The amount of hydroxide radical could reach 11.5 mmol g(-1) (wet) after the beads were catalyzed by NaOH in methanol at 18-20 degreesC for 24 h. The activators such as SESA, glutaraldehyde and p-benzoquinone were used to immobilize penicillin acylase. The penicillin acylase immobilized with SESA exhibited better stability than that with other activators. The successively immobilization could increase the activity of immobilized penicillin acylase about two times and the activity yield 10%, respectively, At the optimal conditions of immobilization, the activity of immobilized penicillin acylase amounted to 812-1200 unit per gram (wet). The kinetics of immobilized penicillin acylase was also studied. The reaction constants, K,, K-iPAA K-iAPA and K-s for immobilized penicillin acylase were equal to 0.033, 0.013, 0.009 and 0.081 mmol/ml, respectively. (C) 2001 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Applied ; Engineering, Chemical ; Polymer Science |
| 研究领域[WOS] | Chemistry ; Engineering ; Polymer Science |
| 关键词[WOS] | AFFINITY LIGANDS ; CHROMATOGRAPHY ; AGAROSE ; ENZYMES |
| 收录类别 | ISTP ; SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000169521800008 |
| 公开日期 | 2013-11-14 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5792]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem Met, Natl Lab Biochem Engn, Beijing 100080, Peoples R China 2.Shandong Univ, Dept Biol Sci, Shandong 270001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, JG,Cong, W,Wang, S,et al. Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase[J]. REACTIVE & FUNCTIONAL POLYMERS,2001,48(1-3):75-84. |
| APA | Liu, JG,Cong, W,Wang, S,&Ouyang, F.(2001).Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase.REACTIVE & FUNCTIONAL POLYMERS,48(1-3),75-84. |
| MLA | Liu, JG,et al."Studies of poly(vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase".REACTIVE & FUNCTIONAL POLYMERS 48.1-3(2001):75-84. |
入库方式： OAI收割
来源：过程工程研究所
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