Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding
文献类型：期刊论文
| 作者 | Gu, ZY; Su, ZG; Janson, JC |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2001-05-25 |
| 卷号 | 918期号:2页码:311-318 |
| 关键词 | gradient elution urea gradient protein refolding lysozyme proteins urea |
| ISSN号 | 0021-9673 |
| 其他题名 | J. Chromatogr. A |
| 中文摘要 | Protein refolding is still a bottleneck for large-scale production of valuable proteins expressed as inclusion bodies in Escherichia coli. Usually biologically active proteins cannot be obtained with high yield at a high concentration after refolding. In order to meet the challenge of protein refolding a urea gradient gel filtration-refolding system was developed in this article. A Superdex 75 column was pre-equilibrated with a linear decreased urea gradient, the denatured protein experienced the gradual decrease in urea concentration as it went through the column. The refolding of denatured lysozyme showed this method could significantly increase the activity recovery of denatured lysozyme at high protein concentration. The activity recovery of 90% was obtained from the initial protein concentration up to 17 mg/ml within 40 min. (C) 2001 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | Protein refolding is still a bottleneck for large-scale production of valuable proteins expressed as inclusion bodies in Escherichia coli. Usually biologically active proteins cannot be obtained with high yield at a high concentration after refolding. In order to meet the challenge of protein refolding a urea gradient gel filtration-refolding system was developed in this article. A Superdex 75 column was pre-equilibrated with a linear decreased urea gradient, the denatured protein experienced the gradual decrease in urea concentration as it went through the column. The refolding of denatured lysozyme showed this method could significantly increase the activity recovery of denatured lysozyme at high protein concentration. The activity recovery of 90% was obtained from the initial protein concentration up to 17 mg/ml within 40 min. (C) 2001 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | INCLUSION-BODIES ; PROTEINS ; RENATURATION ; PURIFICATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000168862400006 |
| 公开日期 | 2013-11-14 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5803]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Uppsala Univ, Biomed Ctr, Uppsala, Sweden |
| 推荐引用方式 GB/T 7714 | Gu, ZY,Su, ZG,Janson, JC. Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding[J]. JOURNAL OF CHROMATOGRAPHY A,2001,918(2):311-318. |
| APA | Gu, ZY,Su, ZG,&Janson, JC.(2001).Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding.JOURNAL OF CHROMATOGRAPHY A,918(2),311-318. |
| MLA | Gu, ZY,et al."Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding".JOURNAL OF CHROMATOGRAPHY A 918.2(2001):311-318. |
入库方式： OAI收割
来源：过程工程研究所
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