中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Activity of tyrosol glucosyltransferase and improved salidroside production through biotransformation of tyrosol in Rhodiola sachalinensis cell cultures

文献类型:期刊论文

作者Xu, JF; Su, ZG; Feng, PS
刊名JOURNAL OF BIOTECHNOLOGY
出版日期1998-03-26
卷号61期号:1页码:69-73
关键词Rhodiola sachalinensis salidroside tyrosol glucosyltransferase biotransformation
ISSN号0168-1656
其他题名J. Biotechnol.
中文摘要The activity of tyrosol glucosyltransferase (TGase) of Rhodiola sachalinensis cells was found to be the highest at the exponential growth phase while the intracellular tyrosol accumulated in large amounts at the stationary growth phase. This non-synchronization of the TGase activity with the tyrosol accumulation was responsible for the low yield of salidroside obtained in cell suspension cultures. When 1 mmol l(-1) exogenous tyrosol was added to the culture at day 8 and subsequently incubated for 24 h, nearly 95% of the added tyrosol was transformed to salidroside. The optimal concentration of exogenous tyrosol for the glucosylation reaction was 3 mmol l(-1). Excess high concentration of tyrosol was toxic to cell growth and inhibited the biotransformation reaction. By repeated addition of 3 mmol l(-1) tyrosol at 24 h intervals over 3 d, very high salidroside yield of 516 mu mol g(-1) (15.4% of dry weight) was obtained. (C) 1998 Elsevier Science B.V. All rights reserved.
英文摘要The activity of tyrosol glucosyltransferase (TGase) of Rhodiola sachalinensis cells was found to be the highest at the exponential growth phase while the intracellular tyrosol accumulated in large amounts at the stationary growth phase. This non-synchronization of the TGase activity with the tyrosol accumulation was responsible for the low yield of salidroside obtained in cell suspension cultures. When 1 mmol l(-1) exogenous tyrosol was added to the culture at day 8 and subsequently incubated for 24 h, nearly 95% of the added tyrosol was transformed to salidroside. The optimal concentration of exogenous tyrosol for the glucosylation reaction was 3 mmol l(-1). Excess high concentration of tyrosol was toxic to cell growth and inhibited the biotransformation reaction. By repeated addition of 3 mmol l(-1) tyrosol at 24 h intervals over 3 d, very high salidroside yield of 516 mu mol g(-1) (15.4% of dry weight) was obtained. (C) 1998 Elsevier Science B.V. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]SUSPENSION-CULTURES ; HYDROQUINONE ; GLUCOSYLATION
收录类别SCI
原文出处://WOS:000074236900006
语种英语
WOS记录号WOS:000074236900006
公开日期2013-11-15
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/5851]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Chem Met, Beijing 100080, Peoples R China
2.Dalian Univ Technol, Inst Biochem Engn, Dalian 116012, Peoples R China
推荐引用方式
GB/T 7714
Xu, JF,Su, ZG,Feng, PS. Activity of tyrosol glucosyltransferase and improved salidroside production through biotransformation of tyrosol in Rhodiola sachalinensis cell cultures[J]. JOURNAL OF BIOTECHNOLOGY,1998,61(1):69-73.
APA Xu, JF,Su, ZG,&Feng, PS.(1998).Activity of tyrosol glucosyltransferase and improved salidroside production through biotransformation of tyrosol in Rhodiola sachalinensis cell cultures.JOURNAL OF BIOTECHNOLOGY,61(1),69-73.
MLA Xu, JF,et al."Activity of tyrosol glucosyltransferase and improved salidroside production through biotransformation of tyrosol in Rhodiola sachalinensis cell cultures".JOURNAL OF BIOTECHNOLOGY 61.1(1998):69-73.

入库方式: OAI收割

来源:过程工程研究所

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