Activity of tyrosol glucosyltransferase and improved salidroside production through biotransformation of tyrosol in Rhodiola sachalinensis cell cultures
文献类型：期刊论文
| 作者 | Xu, JF; Su, ZG; Feng, PS |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 1998-03-26 |
| 卷号 | 61期号:1页码:69-73 |
| 关键词 | Rhodiola sachalinensis salidroside tyrosol glucosyltransferase biotransformation |
| ISSN号 | 0168-1656 |
| 其他题名 | J. Biotechnol. |
| 中文摘要 | The activity of tyrosol glucosyltransferase (TGase) of Rhodiola sachalinensis cells was found to be the highest at the exponential growth phase while the intracellular tyrosol accumulated in large amounts at the stationary growth phase. This non-synchronization of the TGase activity with the tyrosol accumulation was responsible for the low yield of salidroside obtained in cell suspension cultures. When 1 mmol l(-1) exogenous tyrosol was added to the culture at day 8 and subsequently incubated for 24 h, nearly 95% of the added tyrosol was transformed to salidroside. The optimal concentration of exogenous tyrosol for the glucosylation reaction was 3 mmol l(-1). Excess high concentration of tyrosol was toxic to cell growth and inhibited the biotransformation reaction. By repeated addition of 3 mmol l(-1) tyrosol at 24 h intervals over 3 d, very high salidroside yield of 516 mu mol g(-1) (15.4% of dry weight) was obtained. (C) 1998 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | The activity of tyrosol glucosyltransferase (TGase) of Rhodiola sachalinensis cells was found to be the highest at the exponential growth phase while the intracellular tyrosol accumulated in large amounts at the stationary growth phase. This non-synchronization of the TGase activity with the tyrosol accumulation was responsible for the low yield of salidroside obtained in cell suspension cultures. When 1 mmol l(-1) exogenous tyrosol was added to the culture at day 8 and subsequently incubated for 24 h, nearly 95% of the added tyrosol was transformed to salidroside. The optimal concentration of exogenous tyrosol for the glucosylation reaction was 3 mmol l(-1). Excess high concentration of tyrosol was toxic to cell growth and inhibited the biotransformation reaction. By repeated addition of 3 mmol l(-1) tyrosol at 24 h intervals over 3 d, very high salidroside yield of 516 mu mol g(-1) (15.4% of dry weight) was obtained. (C) 1998 Elsevier Science B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SUSPENSION-CULTURES ; HYDROQUINONE ; GLUCOSYLATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000074236900006 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5851]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Chem Met, Beijing 100080, Peoples R China 2.Dalian Univ Technol, Inst Biochem Engn, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, JF,Su, ZG,Feng, PS. Activity of tyrosol glucosyltransferase and improved salidroside production through biotransformation of tyrosol in Rhodiola sachalinensis cell cultures[J]. JOURNAL OF BIOTECHNOLOGY,1998,61(1):69-73. |
| APA | Xu, JF,Su, ZG,&Feng, PS.(1998).Activity of tyrosol glucosyltransferase and improved salidroside production through biotransformation of tyrosol in Rhodiola sachalinensis cell cultures.JOURNAL OF BIOTECHNOLOGY,61(1),69-73. |
| MLA | Xu, JF,et al."Activity of tyrosol glucosyltransferase and improved salidroside production through biotransformation of tyrosol in Rhodiola sachalinensis cell cultures".JOURNAL OF BIOTECHNOLOGY 61.1(1998):69-73. |
入库方式： OAI收割
来源：过程工程研究所
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