Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye
文献类型：期刊论文
| 作者 | Zhang, TX; Liu, HZ; Chen, JY |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 1999-09-01 |
| 卷号 | 4期号:1页码:17-21 |
| 关键词 | affinity extraction reversed micelles Cibacron Blue 3GA bovine serum albumin |
| ISSN号 | 1369-703X |
| 其他题名 | Biochem. Eng. J. |
| 中文摘要 | The affinity extraction of proteins with unbound triazine dye in the mixed reversed micellar system has been studied. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. The affinity CB in the aqueous phase was directly transferred to the mixed reversed micelles formed with cationic surfactant cetyltrimethylammonium bromide (CTAB) and tributyl phosphate (TBP). The transfer of BSA to the reversed micelles increases significantly by the biospecific interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH < pI, the selectivity of proteins can be expected with the presence of affinity CB since no BSA was found to be extracted into the reverse micelles without the addition of CB. The BSA transfer to the reversed micelles increases significantly by the addition of TBP to the organic phase. Most of BSA (similar to 82%) extracted into the reversed micelles can be back extracted into the aqueous phase by the increase of n-hexanol concentration in the organic phase while no CB would be transferred into the stripping aqueous solution at the same time. (C) 1999 Elsevier Science S.A. All rights reserved. |
| 英文摘要 | The affinity extraction of proteins with unbound triazine dye in the mixed reversed micellar system has been studied. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. The affinity CB in the aqueous phase was directly transferred to the mixed reversed micelles formed with cationic surfactant cetyltrimethylammonium bromide (CTAB) and tributyl phosphate (TBP). The transfer of BSA to the reversed micelles increases significantly by the biospecific interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH < pI, the selectivity of proteins can be expected with the presence of affinity CB since no BSA was found to be extracted into the reverse micelles without the addition of CB. The BSA transfer to the reversed micelles increases significantly by the addition of TBP to the organic phase. Most of BSA (similar to 82%) extracted into the reversed micelles can be back extracted into the aqueous phase by the increase of n-hexanol concentration in the organic phase while no CB would be transferred into the stripping aqueous solution at the same time. (C) 1999 Elsevier Science S.A. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | PROTEIN EXTRACTION ; COSURFACTANT ; PURIFICATION ; SEPARATION ; ENZYMES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000082915200003 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5853]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Young Scientist Lab Separat Sci & Engn, Inst Chem Met, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, TX,Liu, HZ,Chen, JY. Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye[J]. BIOCHEMICAL ENGINEERING JOURNAL,1999,4(1):17-21. |
| APA | Zhang, TX,Liu, HZ,&Chen, JY.(1999).Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye.BIOCHEMICAL ENGINEERING JOURNAL,4(1),17-21. |
| MLA | Zhang, TX,et al."Affinity extraction of BSA by mixed reversed micellar system with unbound triazine dye".BIOCHEMICAL ENGINEERING JOURNAL 4.1(1999):17-21. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。