Affinity-based reversed micellar bovine serum albumin (BSA) extraction with unbound reactive dye
文献类型：期刊论文
| 作者 | Zhang, TX; Liu, HZ; Chen, JY |
| 刊名 | SEPARATION SCIENCE AND TECHNOLOGY
 |
| 出版日期 | 2000 |
| 卷号 | 35期号:1页码:143-151 |
| 关键词 | affinity extraction reversed micelles Cibacron Blue 3GA bovine serum albumin |
| ISSN号 | 0149-6395 |
| 其他题名 | Sep. Sci. Technol. |
| 中文摘要 | A quick and simple method has been introduced for affinity extraction with an unbound reactive dye in a reversed micellar system. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. CB in the aqueous phase was directly transferred to the reversed micelles due to electrostatic interaction between anionic CB and cationic cetyltrimethylammonium bromide. The BSA transfer to the reversed micelles increases significantly by affinity interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH close to pI, the selectivity of proteins can be expected in the presence of affinity CB as very little BSA is extracted into the reverse micelles without the addition of CB. Most of the BSA (similar to 82%) extracted into the reversed micelles can be backextracted into the stripping aqueous phase by increasing of n-hexanol concentration in the organic phase while no CB is transferred to the aqueous solution at the same time. |
| 英文摘要 | A quick and simple method has been introduced for affinity extraction with an unbound reactive dye in a reversed micellar system. Bovine serum albumin (BSA) and Cibacron Blue 3GA (CB) were chosen, respectively, as a model protein and an unbound ligand. CB in the aqueous phase was directly transferred to the reversed micelles due to electrostatic interaction between anionic CB and cationic cetyltrimethylammonium bromide. The BSA transfer to the reversed micelles increases significantly by affinity interaction with the addition of a small amount of CB to the aqueous phase. For solutions with pH close to pI, the selectivity of proteins can be expected in the presence of affinity CB as very little BSA is extracted into the reverse micelles without the addition of CB. Most of the BSA (similar to 82%) extracted into the reversed micelles can be backextracted into the stripping aqueous phase by increasing of n-hexanol concentration in the organic phase while no CB is transferred to the aqueous solution at the same time. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Chemistry ; Engineering |
| 关键词[WOS] | PROTEIN EXTRACTION ; NONIONIC SURFACTANT ; BIOAFFINITY ; SEPARATION ; CHROMATOGRAPHY ; COSURFACTANT ; PURIFICATION ; PERFORMANCE ; TRYPSIN ; SYSTEMS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000084758900009 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5855]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Chem Met, Young Scientist Lab Separat Sci & Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, TX,Liu, HZ,Chen, JY. Affinity-based reversed micellar bovine serum albumin (BSA) extraction with unbound reactive dye[J]. SEPARATION SCIENCE AND TECHNOLOGY,2000,35(1):143-151. |
| APA | Zhang, TX,Liu, HZ,&Chen, JY.(2000).Affinity-based reversed micellar bovine serum albumin (BSA) extraction with unbound reactive dye.SEPARATION SCIENCE AND TECHNOLOGY,35(1),143-151. |
| MLA | Zhang, TX,et al."Affinity-based reversed micellar bovine serum albumin (BSA) extraction with unbound reactive dye".SEPARATION SCIENCE AND TECHNOLOGY 35.1(2000):143-151. |
入库方式： OAI收割
来源：过程工程研究所
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