Changes in hydrolysis specificities of lipase from Rhizomucor miehei to polyunsaturated fatty acyl ethyl esters in different aggregation states
文献类型：期刊论文
| 作者 | Kosugi, Y. ; Chang, Q. L. ; Kanazawa, K. ; Nakanishi, H. |
| 刊名 | Journal of the American Oil Chemists Society
 |
| 出版日期 | 1997 |
| 期号 | 11页码:1395-1399 |
| 关键词 | aggregation fatty acid immobilized lipase interfacial activation lipase polyunsaturated fatty acid specificity organic media interfacial activation acids oil immobilization |
| 其他题名 | J. Am. Oil Chem. Soc. |
| 中文摘要 | Hydrolysis specificities of lipase from Rhizomucor miehei were compared for various fatty acyl ethyl esters. Activity yields of immobilized lipases, measured with 1 mM substrate, were more than 100%. Differences in hydrolysis rate and affinity for the substrates between lipase preparations were also typically higher during hydrolysis of substrates at 100 mM than at 1 mM, indicating better mass transfer effects for 1-mM substrates. The native lipase showed higher affinity for polyunsaturated fatty acid substrates at 1 mM than at 100 mM. Hydrolysis rates for 1-mM substrates were observed with immobilized lipases, fixed on anion exchange resin with glutaraldehyde and on cation exchange carrier with carbodiimide, and suggested some modification of the basic amino acid related to the lid of R. miehei lipase. Activation with these bifunctional reagents was not observed for 100-mM substrates, indicating that interfacial activation always occurred because of aggregation of 100-mM substrates. These results show that lipase from R. miehei recognizes molecular aggregation of lipids, and that various changes occur in the hydrolysis specificities for fatty acids. |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5872]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Kosugi, Y.,Chang, Q. L.,Kanazawa, K.,et al. Changes in hydrolysis specificities of lipase from Rhizomucor miehei to polyunsaturated fatty acyl ethyl esters in different aggregation states[J]. Journal of the American Oil Chemists Society,1997(11):1395-1399. |
| APA | Kosugi, Y.,Chang, Q. L.,Kanazawa, K.,&Nakanishi, H..(1997).Changes in hydrolysis specificities of lipase from Rhizomucor miehei to polyunsaturated fatty acyl ethyl esters in different aggregation states.Journal of the American Oil Chemists Society(11),1395-1399. |
| MLA | Kosugi, Y.,et al."Changes in hydrolysis specificities of lipase from Rhizomucor miehei to polyunsaturated fatty acyl ethyl esters in different aggregation states".Journal of the American Oil Chemists Society .11(1997):1395-1399. |
入库方式： OAI收割
来源：过程工程研究所
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