EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES
文献类型：期刊论文
| 作者 | Chang, Q. L. ; Chen, J. Y. |
| 刊名 | Separation Science and Technology
 |
| 出版日期 | 1995 |
| 期号 | 13页码:2679-2693 |
| 关键词 | reversed micelles cosolvent separation alpha-amylase neutral protease liquid-liquid-extraction alpha-chymotrypsin solubilization recovery |
| 其他题名 | Sep. Sci. Technol. |
| 中文摘要 | Seven kinds of alkyl alcohol (n-butanol, n-pentanol, n-hexanol, n-heptanol, n-octanol, n-nonanol, and n-decanol) were chosen as the cosolvent for Aliquat 336/ isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles as the extractant, the separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. Experiments revealed that when n-butanol is used as the cosolvent, the two enzymes from Bacillus subtilis can be effectively separated and purified after a full forward and backward extraction cycle. alpha-Amylase was separated in the stripping solution with about 85% of its total activity recovered and purified about 1.6-fold. Neutral protease was separated in the raffinate with about 80% of its total activity recovered and purified about 3.5-fold. For the other six alcohols used as the cosolvent for Aliquat 336/isooctane reversed micelles, separation was not achieved. |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5901]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Chang, Q. L.,Chen, J. Y.. EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES[J]. Separation Science and Technology,1995(13):2679-2693. |
| APA | Chang, Q. L.,&Chen, J. Y..(1995).EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES.Separation Science and Technology(13),2679-2693. |
| MLA | Chang, Q. L.,et al."EFFECT OF THE TYPE OF COSOLVENT ON THE EXTRACTION PROCESS FOR SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES".Separation Science and Technology .13(1995):2679-2693. |
入库方式： OAI收割
来源：过程工程研究所
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