中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Enhanced salidroside production in liquid-cultivated compact callus aggregates of Rhodiola sachalinensis: manipulation of plant growth regulators and sucrose

文献类型:期刊论文

作者Xu, JF; Ying, PQ; Han, AM; Su, ZG
刊名PLANT CELL TISSUE AND ORGAN CULTURE
出版日期1999
卷号55期号:1页码:53-58
关键词Chinese herb differentiation secondary metabolites
ISSN号0167-6857
其他题名Plant Cell Tissue Organ Cult.
中文摘要Liquid-cultivated compact callus aggregates (CCA) of Rhodiola sachalinensis were initiated from the explant of stems. Microscopic analysis showed that CCA displayed some level of cellular/tissue differentiation, which was perhaps responsible for the increased salidroside production. A higher ratio of cytokinin/auxin was found to be beneficial both for CCA growth and salidroside accumulation. Sucrose (< 100 g l(-1)) had a positive effect on salidroside synthesis, which was not due to an osmotic effect of the medium. The medium containing 40 g l(-1) sucrose, 1.5 mu M naphthaleneacetic acid and 15 mu M 6-benzylaminopurine yielded ca. 120 mg l(-1) salidroside after 24 days of culture.
英文摘要Liquid-cultivated compact callus aggregates (CCA) of Rhodiola sachalinensis were initiated from the explant of stems. Microscopic analysis showed that CCA displayed some level of cellular/tissue differentiation, which was perhaps responsible for the increased salidroside production. A higher ratio of cytokinin/auxin was found to be beneficial both for CCA growth and salidroside accumulation. Sucrose (< 100 g l(-1)) had a positive effect on salidroside synthesis, which was not due to an osmotic effect of the medium. The medium containing 40 g l(-1) sucrose, 1.5 mu M naphthaleneacetic acid and 15 mu M 6-benzylaminopurine yielded ca. 120 mg l(-1) salidroside after 24 days of culture.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Plant Sciences
研究领域[WOS]Biotechnology & Applied Microbiology ; Plant Sciences
关键词[WOS]CELL-SUSPENSION-CULTURES ; ROSMARINIC ACID ; COLEUS-BLUMEI
收录类别SCI
原文出处://WOS:000080431100008
语种英语
WOS记录号WOS:000080431100008
公开日期2013-11-15
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/5919]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Dalian Univ Technol, Inst Biochem Engn, Dalian 116012, Peoples R China
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GB/T 7714
Xu, JF,Ying, PQ,Han, AM,et al. Enhanced salidroside production in liquid-cultivated compact callus aggregates of Rhodiola sachalinensis: manipulation of plant growth regulators and sucrose[J]. PLANT CELL TISSUE AND ORGAN CULTURE,1999,55(1):53-58.
APA Xu, JF,Ying, PQ,Han, AM,&Su, ZG.(1999).Enhanced salidroside production in liquid-cultivated compact callus aggregates of Rhodiola sachalinensis: manipulation of plant growth regulators and sucrose.PLANT CELL TISSUE AND ORGAN CULTURE,55(1),53-58.
MLA Xu, JF,et al."Enhanced salidroside production in liquid-cultivated compact callus aggregates of Rhodiola sachalinensis: manipulation of plant growth regulators and sucrose".PLANT CELL TISSUE AND ORGAN CULTURE 55.1(1999):53-58.

入库方式: OAI收割

来源:过程工程研究所

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