Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB
文献类型：期刊论文
| 作者 | Zhang, TX; Liu, HZ; Chen, JY |
| 刊名 | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2000-09-01 |
| 卷号 | 75期号:9页码:798-802 |
| 关键词 | reversed micelles alcohol dehydrogenase protein purification CTAB |
| ISSN号 | 0268-2575 |
| 其他题名 | J. Chem. Technol. Biotechnol. |
| 中文摘要 | A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified fi om baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was similar to 90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry. |
| 英文摘要 | A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified fi om baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was similar to 90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Chemistry ; Engineering |
| 关键词[WOS] | PROTEIN EXTRACTION ; PHOSPHORIC-ACID ; PURIFICATION ; ENZYMES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000088934100006 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5935]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Lab Separat Sci & Engn, Inst Chem Met, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, TX,Liu, HZ,Chen, JY. Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2000,75(9):798-802. |
| APA | Zhang, TX,Liu, HZ,&Chen, JY.(2000).Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,75(9),798-802. |
| MLA | Zhang, TX,et al."Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 75.9(2000):798-802. |
入库方式： OAI收割
来源：过程工程研究所
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