Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4 center dot 7H(2)O aqueous two-phase system to produce nisin
文献类型：期刊论文
| 作者 | Li, C; Fan, OY; Bai, JH |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2000-05-01 |
| 卷号 | 22期号:10页码:843-847 |
| 关键词 | aqueous two-phase systems extractive cultivation lactic acid L. lactis nisin |
| ISSN号 | 0141-5492 |
| 其他题名 | Biotechnol. Lett. |
| 中文摘要 | To relieve lactic acid inhibition, an aqueous two-phase system (ATPS) was used to grow Lactococcus lactis. Its composition was 11% (w/v) PEG 20000/3.5% (w/v) MgSO4 . 7H(2)O. In this ATPS medium, the cells were completely partitioned in the bottom phase, and lactic acid had the biggest partition coefficient of the eight ATPS media tested. The cell biomass in this medium was 0.64 mg ml(-1), only 60% of that of the control medium, but nisin production (803 IU ml(-1)) was enhanced by 33%. The increase in nisin was explained as a result of extraction of lactic acid from the bottom phase to the top one. The changes of tie-line length and phase volume ratio for the identical tie line could affect cell growth and nisin accumulation. |
| 英文摘要 | To relieve lactic acid inhibition, an aqueous two-phase system (ATPS) was used to grow Lactococcus lactis. Its composition was 11% (w/v) PEG 20000/3.5% (w/v) MgSO4 . 7H(2)O. In this ATPS medium, the cells were completely partitioned in the bottom phase, and lactic acid had the biggest partition coefficient of the eight ATPS media tested. The cell biomass in this medium was 0.64 mg ml(-1), only 60% of that of the control medium, but nisin production (803 IU ml(-1)) was enhanced by 33%. The increase in nisin was explained as a result of extraction of lactic acid from the bottom phase to the top one. The changes of tie-line length and phase volume ratio for the identical tie line could affect cell growth and nisin accumulation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | 2-PHASE SYSTEM ; ALKALINE PROTEASE ; ACID BACTERIA |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000088034000006 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5936]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Hubei Univ, Sch Life Sci, Wuhan 430062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, C,Fan, OY,Bai, JH. Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4 center dot 7H(2)O aqueous two-phase system to produce nisin[J]. BIOTECHNOLOGY LETTERS,2000,22(10):843-847. |
| APA | Li, C,Fan, OY,&Bai, JH.(2000).Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4 center dot 7H(2)O aqueous two-phase system to produce nisin.BIOTECHNOLOGY LETTERS,22(10),843-847. |
| MLA | Li, C,et al."Extractive cultivation of Lactococcus lactis using a polyethylene glycol/MgSO4 center dot 7H(2)O aqueous two-phase system to produce nisin".BIOTECHNOLOGY LETTERS 22.10(2000):843-847. |
入库方式： OAI收割
来源：过程工程研究所
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