High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng
文献类型：期刊论文
| 作者 | Tang, W |
| 刊名 | PLANT CELL REPORTS
 |
| 出版日期 | 2000-06-01 |
| 卷号 | 19期号:7页码:727-732 |
| ISSN号 | 0721-7714 |
| 关键词 | Panax ginseng CA Meyer somatic embryogenesis organogenesis in vitro flowering of regenerated plantlets |
| 其他题名 | Plant Cell Reports |
| 中文摘要 | The morphogenesis ability of light yellowish globular callus derived from cotyledons of mature zygotic embryos of Panax ginseng was investigated. The optimal media for somatic embryogenesis and shoot organogenesis were MS medium containing 0.5 mg l(-1) 2.4-dichlorophenoxyacetic acid, 0.1 mg l(-1) 6-benzyladenine (BA), and 500 mg l(-1) lactoalbumin hydrolysate, and SH medium supplemented with 0.5 mg l(-1) alpha-naphthaleneacetic acid, 0.1 mg l(-1) BA, and 500 mg l(-1) casein hydrolysate. The influences of glucose, mannose, fructose, and sorbose in the media on somatic embryogenesis and shoot organogenesis were revealed as differences in the numbers of somatic embryos and adventitious shoots per gram of morphogenic callus. The best regeneration of somatic embryos was obtained on medium containing glucose, with a mean of 8.7 somatic embryos per gram of callus. The best regeneration of shoots was observed on medium containing fructose, with an average of 12.2 adventitious shoots per gram of callus. Of the somatic embryos 95% were converted into regenerated plantlets, and 100% of adventitious shoots rooted to form regenerated plantlets. Regenerated plants were successfully established in soil. Flowering was observed in 5.7% of the regenerated plants derived from shoot organogenesis and in 1.4% of the regenerated plants derived from somatic embryogenesis. |
| 英文摘要 | The morphogenesis ability of light yellowish globular callus derived from cotyledons of mature zygotic embryos of Panax ginseng was investigated. The optimal media for somatic embryogenesis and shoot organogenesis were MS medium containing 0.5 mg l(-1) 2.4-dichlorophenoxyacetic acid, 0.1 mg l(-1) 6-benzyladenine (BA), and 500 mg l(-1) lactoalbumin hydrolysate, and SH medium supplemented with 0.5 mg l(-1) alpha-naphthaleneacetic acid, 0.1 mg l(-1) BA, and 500 mg l(-1) casein hydrolysate. The influences of glucose, mannose, fructose, and sorbose in the media on somatic embryogenesis and shoot organogenesis were revealed as differences in the numbers of somatic embryos and adventitious shoots per gram of morphogenic callus. The best regeneration of somatic embryos was obtained on medium containing glucose, with a mean of 8.7 somatic embryos per gram of callus. The best regeneration of shoots was observed on medium containing fructose, with an average of 12.2 adventitious shoots per gram of callus. Of the somatic embryos 95% were converted into regenerated plantlets, and 100% of adventitious shoots rooted to form regenerated plantlets. Regenerated plants were successfully established in soil. Flowering was observed in 5.7% of the regenerated plants derived from shoot organogenesis and in 1.4% of the regenerated plants derived from somatic embryogenesis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | CELL-CULTURES ; SAPONIN PRODUCTION ; EMBRYOS ; GROWTH |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000088156600014 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5956]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Chem Met, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, W. High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng[J]. PLANT CELL REPORTS,2000,19(7):727-732. |
| APA | Tang, W.(2000).High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng.PLANT CELL REPORTS,19(7),727-732. |
| MLA | Tang, W."High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng".PLANT CELL REPORTS 19.7(2000):727-732. |
入库方式： OAI收割
来源：过程工程研究所
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