The interaction between Triton X-100 and bovine serum albumin
文献类型:期刊论文
| 作者 | Wei, XF; Liu, HZ |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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| 出版日期 | 2000-06-20 |
| 卷号 | 28期号:6页码:699-701 |
| 关键词 | bovine serum albumin fluorimetry |
| ISSN号 | 0253-3820 |
| 其他题名 | Chin. J. Anal. Chem. |
| 中文摘要 | The binding of Triton X-100 (Tx) to bovine serum albumin(BSA) was studied using fluorescence spectroscopy. It was shown that TX has a strong ability to quench the BSA fluorescence mainly via a static quenching. The binding constant K and the number of bindig n were obtained from the fluorescence quenching results. It was revealed that K and n were changed with varying concentrations of TX: below cmc of TX, K was 440 mol/L, n was 0.91; above cmc, K was 10 mg/L, n was 0.42. The hydrophobic interactions played a major role in the binding process. |
| 英文摘要 | The binding of Triton X-100 (Tx) to bovine serum albumin(BSA) was studied using fluorescence spectroscopy. It was shown that TX has a strong ability to quench the BSA fluorescence mainly via a static quenching. The binding constant K and the number of bindig n were obtained from the fluorescence quenching results. It was revealed that K and n were changed with varying concentrations of TX: below cmc of TX, K was 440 mol/L, n was 0.91; above cmc, K was 10 mg/L, n was 0.42. The hydrophobic interactions played a major role in the binding process. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | PROTEIN FLUORESCENCE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000087852500009 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5969] ![]() |
| 专题 | 过程工程研究所_研究所(批量导入) |
| 作者单位 | Chinese Acad Sci, Inst Chem Met, Young Sci Lab Separat Sci & Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, XF,Liu, HZ. The interaction between Triton X-100 and bovine serum albumin[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(6):699-701. |
| APA | Wei, XF,&Liu, HZ.(2000).The interaction between Triton X-100 and bovine serum albumin.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,28(6),699-701. |
| MLA | Wei, XF,et al."The interaction between Triton X-100 and bovine serum albumin".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 28.6(2000):699-701. |
入库方式: OAI收割
来源:过程工程研究所
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