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The interaction between Triton X-100 and bovine serum albumin

文献类型:期刊论文

作者Wei, XF; Liu, HZ
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
出版日期2000-06-20
卷号28期号:6页码:699-701
关键词bovine serum albumin fluorimetry
ISSN号0253-3820
其他题名Chin. J. Anal. Chem.
中文摘要The binding of Triton X-100 (Tx) to bovine serum albumin(BSA) was studied using fluorescence spectroscopy. It was shown that TX has a strong ability to quench the BSA fluorescence mainly via a static quenching. The binding constant K and the number of bindig n were obtained from the fluorescence quenching results. It was revealed that K and n were changed with varying concentrations of TX: below cmc of TX, K was 440 mol/L, n was 0.91; above cmc, K was 10 mg/L, n was 0.42. The hydrophobic interactions played a major role in the binding process.
英文摘要The binding of Triton X-100 (Tx) to bovine serum albumin(BSA) was studied using fluorescence spectroscopy. It was shown that TX has a strong ability to quench the BSA fluorescence mainly via a static quenching. The binding constant K and the number of bindig n were obtained from the fluorescence quenching results. It was revealed that K and n were changed with varying concentrations of TX: below cmc of TX, K was 440 mol/L, n was 0.91; above cmc, K was 10 mg/L, n was 0.42. The hydrophobic interactions played a major role in the binding process.
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PROTEIN FLUORESCENCE
收录类别SCI
原文出处://WOS:000087852500009
语种英语
WOS记录号WOS:000087852500009
公开日期2013-11-15
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/5969]  
专题过程工程研究所_研究所(批量导入)
作者单位Chinese Acad Sci, Inst Chem Met, Young Sci Lab Separat Sci & Engn, Beijing 100080, Peoples R China
推荐引用方式
GB/T 7714
Wei, XF,Liu, HZ. The interaction between Triton X-100 and bovine serum albumin[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(6):699-701.
APA Wei, XF,&Liu, HZ.(2000).The interaction between Triton X-100 and bovine serum albumin.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,28(6),699-701.
MLA Wei, XF,et al."The interaction between Triton X-100 and bovine serum albumin".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 28.6(2000):699-701.

入库方式: OAI收割

来源:过程工程研究所

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