Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors
文献类型：期刊论文
| 作者 | Xu, J. F. ; Xie, J. ; Han, A. M. ; Feng, P. S. ; Su, Z. G. |
| 刊名 | Journal of Chemical Technology and Biotechnology
 |
| 出版日期 | 1998 |
| 期号 | 3页码:227-234 |
| 关键词 | Rhodiola sachalinensis salidroside plant cell culture aggregates air-lift reactor oxygen transfer coefficient plant-cell cultures tissue-cultures growth accumulation root |
| 其他题名 | J. Chem. Technol. Biotechnol. |
| 中文摘要 | Compact callus aggregates (CCA) of Rhodiola sachalinensis, spherical, smooth-surfaced clumps of 2-8 mm in diameter displaying some level of tissue differentiation, were successfully cultured in 10 dm(3) and 100 dm(3) air-lift reactors. High salidroside yields of 60.0 mg dm(-3) were obtained, which were 10-fold the dispersed cell cultures. The salidroside accumulation was found to be growth-associated due to the differentiated structure of CCA. No 'foaming' was observed since the broth remained almost clear throughout the culture cycle. The size of CCA conformed to normal distribution with average diameters varying from 3.1 mm to 3.6 mm during the culture. The depositing velocity of CCA in culture broth was small enough to be readily retained in suspension, therefore avoiding the clogging of the reactors. The significant increase in solid hold-up of the culture system was suggested to contribute to the variation of k(L) a during the culture. (C) 1998 SCI. |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/5974]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Xu, J. F.,Xie, J.,Han, A. M.,et al. Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors[J]. Journal of Chemical Technology and Biotechnology,1998(3):227-234. |
| APA | Xu, J. F.,Xie, J.,Han, A. M.,Feng, P. S.,&Su, Z. G..(1998).Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors.Journal of Chemical Technology and Biotechnology(3),227-234. |
| MLA | Xu, J. F.,et al."Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors".Journal of Chemical Technology and Biotechnology .3(1998):227-234. |
入库方式： OAI收割
来源：过程工程研究所
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