Peroxidase activity of desiccation-tolerant loblolly pine somatic embryos
文献类型:期刊论文
作者 | Tang, W |
刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
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出版日期 | 2000-11-01 |
卷号 | 36期号:6页码:488-491 |
关键词 | Pinus taeda L somatic embryogenesis desiccation tolerance peroxidase activity |
ISSN号 | 1054-5476 |
其他题名 | In Vitro Cell. Dev. Biol.-Plant |
中文摘要 | Desiccation tolerance can be induced by culturing somatic embryos of loblolly pine (Pinus taeda L.) on medium supplemented with 50 muM abscisic acid (ABA) and/or 8.5% polyethylene glycol (PEG(6000)). Scanning electron microscopy of desiccated somatic embryos showed that the size and external morphology of the desiccation-tolerant somatic embryos recovered to the pre-desiccation state within 24-36 h, whereas the non-desiccation-tolerant somatic embryos did not recover and remained shriveled. after rehydration. Peroxidase activity of desiccated somatic embryos increased sharply after 1 d of desiccation treatment at 87% relative humidity (RH), and desiccation-tolerant somatic embryos had higher peroxidase activity compared to sensitive somatic embryos. Higher peroxidase activity of desiccation-tolerant somatic embryos map have allowed them to catalyze the reduction of H(2)O(2) produced by drought stress, and protected them from oxidative damage. |
英文摘要 | Desiccation tolerance can be induced by culturing somatic embryos of loblolly pine (Pinus taeda L.) on medium supplemented with 50 muM abscisic acid (ABA) and/or 8.5% polyethylene glycol (PEG(6000)). Scanning electron microscopy of desiccated somatic embryos showed that the size and external morphology of the desiccation-tolerant somatic embryos recovered to the pre-desiccation state within 24-36 h, whereas the non-desiccation-tolerant somatic embryos did not recover and remained shriveled. after rehydration. Peroxidase activity of desiccated somatic embryos increased sharply after 1 d of desiccation treatment at 87% relative humidity (RH), and desiccation-tolerant somatic embryos had higher peroxidase activity compared to sensitive somatic embryos. Higher peroxidase activity of desiccation-tolerant somatic embryos map have allowed them to catalyze the reduction of H2O2 produced by drought stress, and protected them from oxidative damage. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
研究领域[WOS] | Plant Sciences ; Cell Biology ; Developmental Biology |
关键词[WOS] | SCANNING-ELECTRON-MICROSCOPY ; MICROSPORE-DERIVED EMBRYOS ; HIGH RELATIVE-HUMIDITY ; GLAUCA MOENCH VOSS ; ABSCISIC-ACID ; WHITE SPRUCE ; ZYGOTIC EMBRYOS ; BRASSICA-NAPUS ; RED SPRUCE ; GERMINATION |
收录类别 | SCI |
原文出处 | |
语种 | 英语 |
WOS记录号 | WOS:000167450300008 |
公开日期 | 2013-11-15 |
版本 | 出版稿 |
源URL | [http://ir.ipe.ac.cn/handle/122111/6016] ![]() |
专题 | 过程工程研究所_研究所(批量导入) |
作者单位 | Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
推荐引用方式 GB/T 7714 | Tang, W. Peroxidase activity of desiccation-tolerant loblolly pine somatic embryos[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2000,36(6):488-491. |
APA | Tang, W.(2000).Peroxidase activity of desiccation-tolerant loblolly pine somatic embryos.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,36(6),488-491. |
MLA | Tang, W."Peroxidase activity of desiccation-tolerant loblolly pine somatic embryos".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 36.6(2000):488-491. |
入库方式: OAI收割
来源:过程工程研究所
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