Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine
文献类型:期刊论文
作者 | Tang, W; Ouyang, F; Guo, ZC |
刊名 | PLANT CELL REPORTS
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出版日期 | 1998-04-01 |
卷号 | 17期号:6-7页码:557-560 |
关键词 | Pinus taeda L. organogenesis plant regeneration |
ISSN号 | 0721-7714 |
其他题名 | Plant Cell Reports |
中文摘要 | Mature zygotic embryos from three seed sources of loblolly pine were cultured on callus induction medium containing 10 mg l(-1) alpha-naphthaleneacetic acid, 4 mg l(-1) benzyladenine (BA), 400 mg l(-1) casein hydrolysate, and 400 mg l(-1) glutamine for 6 weeks. Light-yellow, loose, glossy, globular callus was formed, and the highest frequency was 35.7%. The highest differentiation frequency of callus on adventitious bud induction medium was 62.1%. After culture of calli with adventitious buds on elongation medium for 6 weeks, adventitious shoots more than 1.0 cm in height were selected for rooting. On rooting medium supplemented with 0.1 mg l(-1) indole-3-butyric acid, 1 mg l(-1) BA, and 0.5 mg l(-1) gibberellic acid, the highest rooting frequency of adventitious shoots was 46% in a culture period of 6 weeks. Established plants survived following transfer to soil at a frequency of 71%. |
英文摘要 | Mature zygotic embryos from three seed sources of loblolly pine were cultured on callus induction medium containing 10 mg l(-1) alpha-naphthaleneacetic acid, 4 mg l(-1) benzyladenine (BA), 400 mg l(-1) casein hydrolysate, and 400 mg l(-1) glutamine for 6 weeks. Light-yellow, loose, glossy, globular callus was formed, and the highest frequency was 35.7%. The highest differentiation frequency of callus on adventitious bud induction medium was 62.1%. After culture of calli with adventitious buds on elongation medium for 6 weeks, adventitious shoots more than 1.0 cm in height were selected for rooting. On rooting medium supplemented with 0.1 mg l(-1) indole-3-butyric acid, 1 mg l(-1) BA, and 0.5 mg l(-1) gibberellic acid, the highest rooting frequency of adventitious shoots was 46% in a culture period of 6 weeks. Established plants survived following transfer to soil at a frequency of 71%. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Plant Sciences |
研究领域[WOS] | Plant Sciences |
关键词[WOS] | ABSCISIC-ACID ; CULTURES ; TAEDA |
收录类别 | SCI |
原文出处 | |
语种 | 英语 |
WOS记录号 | WOS:000073270200021 |
公开日期 | 2013-11-15 |
版本 | 出版稿 |
源URL | [http://ir.ipe.ac.cn/handle/122111/6019] ![]() |
专题 | 过程工程研究所_研究所(批量导入) |
作者单位 | 1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Tang, W,Ouyang, F,Guo, ZC. Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine[J]. PLANT CELL REPORTS,1998,17(6-7):557-560. |
APA | Tang, W,Ouyang, F,&Guo, ZC.(1998).Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine.PLANT CELL REPORTS,17(6-7),557-560. |
MLA | Tang, W,et al."Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine".PLANT CELL REPORTS 17.6-7(1998):557-560. |
入库方式: OAI收割
来源:过程工程研究所
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