中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine

文献类型:期刊论文

作者Tang, W; Ouyang, F; Guo, ZC
刊名PLANT CELL REPORTS
出版日期1998-04-01
卷号17期号:6-7页码:557-560
关键词Pinus taeda L. organogenesis plant regeneration
ISSN号0721-7714
其他题名Plant Cell Reports
中文摘要Mature zygotic embryos from three seed sources of loblolly pine were cultured on callus induction medium containing 10 mg l(-1) alpha-naphthaleneacetic acid, 4 mg l(-1) benzyladenine (BA), 400 mg l(-1) casein hydrolysate, and 400 mg l(-1) glutamine for 6 weeks. Light-yellow, loose, glossy, globular callus was formed, and the highest frequency was 35.7%. The highest differentiation frequency of callus on adventitious bud induction medium was 62.1%. After culture of calli with adventitious buds on elongation medium for 6 weeks, adventitious shoots more than 1.0 cm in height were selected for rooting. On rooting medium supplemented with 0.1 mg l(-1) indole-3-butyric acid, 1 mg l(-1) BA, and 0.5 mg l(-1) gibberellic acid, the highest rooting frequency of adventitious shoots was 46% in a culture period of 6 weeks. Established plants survived following transfer to soil at a frequency of 71%.
英文摘要Mature zygotic embryos from three seed sources of loblolly pine were cultured on callus induction medium containing 10 mg l(-1) alpha-naphthaleneacetic acid, 4 mg l(-1) benzyladenine (BA), 400 mg l(-1) casein hydrolysate, and 400 mg l(-1) glutamine for 6 weeks. Light-yellow, loose, glossy, globular callus was formed, and the highest frequency was 35.7%. The highest differentiation frequency of callus on adventitious bud induction medium was 62.1%. After culture of calli with adventitious buds on elongation medium for 6 weeks, adventitious shoots more than 1.0 cm in height were selected for rooting. On rooting medium supplemented with 0.1 mg l(-1) indole-3-butyric acid, 1 mg l(-1) BA, and 0.5 mg l(-1) gibberellic acid, the highest rooting frequency of adventitious shoots was 46% in a culture period of 6 weeks. Established plants survived following transfer to soil at a frequency of 71%.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Plant Sciences
研究领域[WOS]Plant Sciences
关键词[WOS]ABSCISIC-ACID ; CULTURES ; TAEDA
收录类别SCI
原文出处://WOS:000073270200021
语种英语
WOS记录号WOS:000073270200021
公开日期2013-11-15
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/6019]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714
Tang, W,Ouyang, F,Guo, ZC. Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine[J]. PLANT CELL REPORTS,1998,17(6-7):557-560.
APA Tang, W,Ouyang, F,&Guo, ZC.(1998).Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine.PLANT CELL REPORTS,17(6-7),557-560.
MLA Tang, W,et al."Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine".PLANT CELL REPORTS 17.6-7(1998):557-560.

入库方式: OAI收割

来源:过程工程研究所

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