Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures
文献类型：期刊论文
| 作者 | Xu, JF; Su, ZG; Feng, PS |
| 刊名 | ACTA BOTANICA SINICA
 |
| 出版日期 | 1998 |
| 卷号 | 40期号:12页码:1129-1135 |
| 关键词 | Rhodiola sachalinensis salidroside tyrosol biotransformation |
| ISSN号 | 0577-7496 |
| 其他题名 | Acta Bot. Sin. |
| 中文摘要 | In Rhodiola sachalinensis A. Bor. cell cultures, low yields of salidroside was supposed to be associated with the low efficiency of glucosylation reaction at the stationary phase of cell growth, when large amounts of the substrate, aglycon tyrosol, were accumulated. Considering the activity of tyrosol glucosyltransferase being the highest at the exponential growth phase, the author added exogenous tyrosol into the cultures at this time so as to produce salidroside through biotransformation. The effects of tyrosol concentration, the way of tyrosol addition as well as the cell density on the transformation rate and salidroside yield were investigated. It was found that the transformation rate attained 95% after cells were incubated in the medium containing 1 mmol/L tyrosol for 24 h. Excess high concentrations of tyrosol in medium (> 3 mmol/L) caused inhibition of transformation rate and cell growth. By 3 repeated additions of tyrosol in low concentrations, the salidroside yields of 1 320 mg/L, 1 740 mg/L and 1 980 mg/L to the cell densities of 6 g DW/L, 12 g DW/L and 18 g DW/L were obtained respectively. |
| 英文摘要 | In Rhodiola sachalinensis A. Bor. cell cultures, low yields of salidroside was supposed to be associated with the low efficiency of glucosylation reaction at the stationary phase of cell growth, when large amounts of the substrate, aglycon tyrosol, were accumulated. Considering the activity of tyrosol glucosyltransferase being the highest at the exponential growth phase, the author added exogenous tyrosol into the cultures at this time so as to produce salidroside through biotransformation. The effects of tyrosol concentration, the way of tyrosol addition as well as the cell density on the transformation rate and salidroside yield were investigated. It was found that the transformation rate attained 95% after cells were incubated in the medium containing 1 mmol/L tyrosol for 24 h. Excess high concentrations of tyrosol in medium (> 3 mmol/L) caused inhibition of transformation rate and cell growth. By 3 repeated additions of tyrosol in low concentrations, the salidroside yields of 1 320 mg/L, 1 740 mg/L and 1 980 mg/L to the cell densities of 6 g DW/L, 12 g DW/L and 18 g DW/L were obtained respectively. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 关键词[WOS] | HYDROQUINONE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000077833800008 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6037]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Dalian Univ Technol, Inst Biochem Engn, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, JF,Su, ZG,Feng, PS. Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures[J]. ACTA BOTANICA SINICA,1998,40(12):1129-1135. |
| APA | Xu, JF,Su, ZG,&Feng, PS.(1998).Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures.ACTA BOTANICA SINICA,40(12),1129-1135. |
| MLA | Xu, JF,et al."Production of salidroside through biotransformation of exogenous tyrosol in Rhodiola sachalinensis cell suspension cultures".ACTA BOTANICA SINICA 40.12(1998):1129-1135. |
入库方式： OAI收割
来源：过程工程研究所
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