PURIFICATION OF INDUSTRIAL ALPHA-AMYLASE BY REVERSED MICELLAR EXTRACTION
文献类型：期刊论文
| 作者 | Chang, Q. L. ; Chen, J. Y. |
| 刊名 | Biotechnology and Bioengineering
 |
| 出版日期 | 1995 |
| 期号 | 6页码:745-748 |
| 关键词 | aliquat 336 reversed micelles purification alpha-amylase liquid-liquid-extraction solubilization recovery |
| 其他题名 | Biotechnol. Bioeng. |
| 中文摘要 | The purification of industrial alpha-amylase by liquid-liquid extraction with Aliquat 336 reversed micellar solution as the extractant was studied. Seven kinds of Aliquat 336 reversed micellar solution, formed by using seven kinds of straight chain alkyl alcohols as cosolvent, have been utilized to extract industrial alpha-amylase. It was found that these seven kinds of reversed micellar solution can all achieve a high protein transfer efficiency in the forward extraction process. After a full forward and backward extraction cycle, however, only the reversed micelles with n-butanol as the cosolvent was found to be able to maintain the activity of alpha-amylase in the stripping solution. By using the reversed micelles of Aliquat 336/isooctane/1% (v/v) n-butanol to perform a full extraction cycle, it was found that 85% of the total activity of alpha-amylase in the industrial alpha-amylase could be recovered at the end of an extraction cycle and the specific activity of alpha-amylase could be concentrated about 1.5-fold; meanwhile, most of the neutral protease in the industrial alpha-amylase could be removed. The separation factor of alpha-amylase to neutral protease at the end of an extraction cycle can reach about 10. (C) 1995 John Wiley & Sons, Inc. |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6041]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Chang, Q. L.,Chen, J. Y.. PURIFICATION OF INDUSTRIAL ALPHA-AMYLASE BY REVERSED MICELLAR EXTRACTION[J]. Biotechnology and Bioengineering,1995(6):745-748. |
| APA | Chang, Q. L.,&Chen, J. Y..(1995).PURIFICATION OF INDUSTRIAL ALPHA-AMYLASE BY REVERSED MICELLAR EXTRACTION.Biotechnology and Bioengineering(6),745-748. |
| MLA | Chang, Q. L.,et al."PURIFICATION OF INDUSTRIAL ALPHA-AMYLASE BY REVERSED MICELLAR EXTRACTION".Biotechnology and Bioengineering .6(1995):745-748. |
入库方式： OAI收割
来源：过程工程研究所
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