Recovery of taxol from the extract of Taxus cuspidate callus cultures with Al2O3 chromatography
文献类型：期刊论文
| 作者 | Zhang, ZQ; Su, ZG |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2000 |
| 卷号 | 23期号:17页码:2683-2693 |
| 关键词 | liquid-chromatography taxanes brevifolia |
| ISSN号 | 1082-6076 |
| 其他题名 | J. Liq. Chromatogr. Relat. Technol. |
| 中文摘要 | In this study, we reported a chromatographic protocol using C-18 silica gel phase and basic Al2O3 phase to research the creating and recovery of taxol from the extract of Taxus cuspidate callus cultures. With the cleaning up by basic Al2O3 chromatography, the content of taxol determined in our experiment was increased greatly. The main source of increasing taxol concentration was the isomerization of 7-epi-taxol to taxol; taxol could also be decomposed to baccatin III and 10-deacetylbaccatin III in the Al2O3 column. At the optimized conditions, the total recovery of taxol from the extract was more than 160%. |
| 英文摘要 | In this study, we reported a chromatographic protocol using C-18 silica gel phase and basic Al2O3 phase to research the creating and recovery of taxol from the extract of Taxus cuspidate callus cultures. With the cleaning up by basic Al2O3 chromatography, the content of taxol determined in our experiment was increased greatly. The main source of increasing taxol concentration was the isomerization of 7-epi-taxol to taxol; taxol could also be decomposed to baccatin III and 10-deacetylbaccatin III in the Al2O3 column. At the optimized conditions, the total recovery of taxol from the extract was more than 160%. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | LIQUID-CHROMATOGRAPHY ; TAXANES ; BREVIFOLIA |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000090063000006 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6046]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, ZQ,Su, ZG. Recovery of taxol from the extract of Taxus cuspidate callus cultures with Al2O3 chromatography[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2000,23(17):2683-2693. |
| APA | Zhang, ZQ,&Su, ZG.(2000).Recovery of taxol from the extract of Taxus cuspidate callus cultures with Al2O3 chromatography.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,23(17),2683-2693. |
| MLA | Zhang, ZQ,et al."Recovery of taxol from the extract of Taxus cuspidate callus cultures with Al2O3 chromatography".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 23.17(2000):2683-2693. |
入库方式： OAI收割
来源：过程工程研究所
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