SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES - STUDY OF THE EFFECT OF COSOLVENT CONCENTRATION
文献类型：期刊论文
| 作者 | Chang, Q. L. ; Chen, J. Y. |
| 刊名 | Chemical Engineering Journal and the Biochemical Engineering Journal
 |
| 出版日期 | 1995 |
| 期号 | 3页码:303-308 |
| 关键词 | reversed micelles cosolvent separation alpha-amylase neutral protease liquid-liquid-extraction alpha-chymotrypsin protein extraction solubilization |
| 其他题名 | Chem. Eng. J. Biochem. Eng. J. |
| 中文摘要 | Two kinds of alkyl alcohol (n-butanol and n-octanol) at concentrations ranging from 0.4% to 2.0% (v/v) were chosen as the cosolvent for Aliquat 336/isooctane reversed micelles. By performing liquid-liquid extraction with Aliquat 336 reversed micelles, separation and purification of two enzymes (alpha-amylase and neutral protease) from Bacillus subtilis were investigated. It was found that at higher cosolvent concentration (above 0.4% v/v), n-butanol may be a suitable cosolvent for Aliquat 336 reversed micelles. Under this condition, about 90% of the total activity of alpha-amylase in the crude enzyme preparation can be recovered in the stripping solution after one extraction cycle and the enzyme can be purified about 1.4-fold; meanwhile about 70% of the total activity of neutral protease in the crude enzyme preparation is retrieved into the raffinate with about 4.5-fold purification. At the lower cosolvent concentration range (below 0.4% v/v), however, n-octanol is the better choice. Under this condition, about 85% of the total activity of alpha-amylase can be recovered into the stripping solution and purified by 1.3-fold; meanwhile about 75% of the total activity of neutral protease is transferred into the raffinate with the enzyme purified by 4.0-fold. |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6059]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Chang, Q. L.,Chen, J. Y.. SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES - STUDY OF THE EFFECT OF COSOLVENT CONCENTRATION[J]. Chemical Engineering Journal and the Biochemical Engineering Journal,1995(3):303-308. |
| APA | Chang, Q. L.,&Chen, J. Y..(1995).SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES - STUDY OF THE EFFECT OF COSOLVENT CONCENTRATION.Chemical Engineering Journal and the Biochemical Engineering Journal(3),303-308. |
| MLA | Chang, Q. L.,et al."SEPARATION AND PURIFICATION OF 2 ENZYMES FROM BACILLUS-SUBTILIS USING ALIQUAT-336 REVERSED MICELLES - STUDY OF THE EFFECT OF COSOLVENT CONCENTRATION".Chemical Engineering Journal and the Biochemical Engineering Journal .3(1995):303-308. |
入库方式： OAI收割
来源：过程工程研究所
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