Simultaneous cell disruption and aqueous two-phase extraction for isolation of intracellular recombinant proteins
文献类型：期刊论文
| 作者 | Wang, GH; Feng, XL; Su, ZG |
| 刊名 | CHINESE JOURNAL OF CHEMICAL ENGINEERING
 |
| 出版日期 | 1999-06-01 |
| 卷号 | 7期号:2页码:139-144 |
| 关键词 | SDATE release of intracellular proteins high speed bead mill aqueous two-phase extraction tumor necrosis factor |
| ISSN号 | 1004-9541 |
| 其他题名 | Chin. J. Chem. Eng. |
| 中文摘要 | A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and preliminary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required. |
| 英文摘要 | A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and preliminary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | TUMOR NECROSIS FACTOR ; PURIFICATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000081096200007 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6064]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Dalian Univ Technol, Biochem Engn Lab, Dalian 116012, Peoples R China 2.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, GH,Feng, XL,Su, ZG. Simultaneous cell disruption and aqueous two-phase extraction for isolation of intracellular recombinant proteins[J]. CHINESE JOURNAL OF CHEMICAL ENGINEERING,1999,7(2):139-144. |
| APA | Wang, GH,Feng, XL,&Su, ZG.(1999).Simultaneous cell disruption and aqueous two-phase extraction for isolation of intracellular recombinant proteins.CHINESE JOURNAL OF CHEMICAL ENGINEERING,7(2),139-144. |
| MLA | Wang, GH,et al."Simultaneous cell disruption and aqueous two-phase extraction for isolation of intracellular recombinant proteins".CHINESE JOURNAL OF CHEMICAL ENGINEERING 7.2(1999):139-144. |
入库方式： OAI收割
来源：过程工程研究所
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