Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production
文献类型：期刊论文
| 作者 | Xu, JF; Su, ZG; Feng, PS |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 1998-07-01 |
| 卷号 | 23期号:1-2页码:20-27 |
| 关键词 | Rhodiola sachalinensis salidroside suspension culture plant cell aggregate |
| ISSN号 | 0141-0229 |
| 其他题名 | Enzyme Microb. Technol. |
| 中文摘要 | Some level of cellular or tissue differentiation is needed for the biosynthesis of many important compounds in plant cell cultures. The culture of compact callus aggregates (CCA) consisting of spherical, smooth-surfaced clumps of 2-7 mm in diameter displaying some level of cellular and tissue differentiation was initiated from the stems of R. sachalinensis. Histochemical analysis showed that the CCA were structurally organized to facilitate nutrient transport. The kinetics for CCA growth and salidroside synthesis were measured in shake flasks. it was found that a short phase of exponential growth (mu = 0.137 day(-1)) and a relatively long phase of linear growth (growth rate = 0.60 g day(-1) l(-1) predominated over most of the the culture cycle. The salidroside yield of CCA after 24 days of culture was 0.68% on a dry weight basis, approximately six times as much as that of the dispersed cells; however, the growth rate of CCA was comparable to that of the dispersed cells. The CCA suspension culture was also successfully carried out ill a 5-l airlift bioreactor. The yield of salidroside was similar to that of shake-flask cultures though the biomass was relatively small. No foam buildup occurred in the reactor Since rite medium remained almost clear throughout the culture cycle. (C) 1998 Elsevier Science Inc. |
| 英文摘要 | Some level of cellular or tissue differentiation is needed for the biosynthesis of many important compounds in plant cell cultures. The culture of compact callus aggregates (CCA) consisting of spherical, smooth-surfaced clumps of 2-7 mm in diameter displaying some level of cellular and tissue differentiation was initiated from the stems of R. sachalinensis. Histochemical analysis showed that the CCA were structurally organized to facilitate nutrient transport. The kinetics for CCA growth and salidroside synthesis were measured in shake flasks. it was found that a short phase of exponential growth (mu = 0.137 day(-1)) and a relatively long phase of linear growth (growth rate = 0.60 g day(-1) l(-1) predominated over most of the the culture cycle. The salidroside yield of CCA after 24 days of culture was 0.68% on a dry weight basis, approximately six times as much as that of the dispersed cells; however, the growth rate of CCA was comparable to that of the dispersed cells. The CCA suspension culture was also successfully carried out ill a 5-l airlift bioreactor. The yield of salidroside was similar to that of shake-flask cultures though the biomass was relatively small. No foam buildup occurred in the reactor Since rite medium remained almost clear throughout the culture cycle. (C) 1998 Elsevier Science Inc. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | PLANT-CELL CULTURES ; FED-BATCH CULTURE ; HAIRY ROOT ; GROWTH |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000074839200004 |
| 公开日期 | 2013-11-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6088]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Dalian Univ Technol, Inst Biochem Engn, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, JF,Su, ZG,Feng, PS. Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production[J]. ENZYME AND MICROBIAL TECHNOLOGY,1998,23(1-2):20-27. |
| APA | Xu, JF,Su, ZG,&Feng, PS.(1998).Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production.ENZYME AND MICROBIAL TECHNOLOGY,23(1-2),20-27. |
| MLA | Xu, JF,et al."Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production".ENZYME AND MICROBIAL TECHNOLOGY 23.1-2(1998):20-27. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。