Preconcentration of 3-nitrotyrosine in urine by transient isotachophoresis in MEKC
文献类型：期刊论文
| 作者 | Ren HX(任海霞)1,2,3 ; Liu X(刘霞)1,2; Jiang SX(蒋生祥)1,2
|
| 刊名 | Journal of Pharmaceutical and Biomedical Analysis
 |
| 出版日期 | 2013 |
| 卷号 | 78-79页码:100-104 |
| 关键词 | Capillary electrophoresis CE-UV High-salt stacking Nitrotyrosine |
| ISSN号 | 0731-7085 |
| 通讯作者 | 蒋生祥 |
| 英文摘要 | Transient isotachophoresis (tITP), usually performed in CZE, is a useful on-line sample preconcentration technique for high saline samples. However, only a few papers have applied tITP in MEKC especially SDS system. This study compared tITP in MEKC with that in CZE for 3-nitro-tyrosine (NT) analysis in urine. No sample clean-up was required. Self-stacking occurred simultaneously as chloride in urine acted as a leading ion. HEPES-Tris buffer at pH 8.2 was used as a terminating electrolyte and separation buffer. UV detection wavelength was set at 426 nm. Experiments showed that tITP in MEKC exhibited much higher peak efficiency (up to 1 000 000), and improved 4-fold sensitivity, compared with those by tITP in CZE. The limit of detection was 0.07 mu M for 3-nitrotyrosine in urine. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the Natural Science Foundation of Gansu province, China (No. 0803RJZ017);the National Science & Technology Major Project, China (2011ZX05010;2011ZX05011004) |
| 语种 | 英语 |
| WOS记录号 | WOS:000317442800015 |
| 公开日期 | 2013-12-10 |
| 源URL | [http://210.77.64.217/handle/362003/4619]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, CAS, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren HX,Liu X,Jiang SX. Preconcentration of 3-nitrotyrosine in urine by transient isotachophoresis in MEKC[J]. Journal of Pharmaceutical and Biomedical Analysis,2013,78-79:100-104. |
| APA | Ren HX,Liu X,&Jiang SX.(2013).Preconcentration of 3-nitrotyrosine in urine by transient isotachophoresis in MEKC.Journal of Pharmaceutical and Biomedical Analysis,78-79,100-104. |
| MLA | Ren HX,et al."Preconcentration of 3-nitrotyrosine in urine by transient isotachophoresis in MEKC".Journal of Pharmaceutical and Biomedical Analysis 78-79(2013):100-104. |
入库方式： OAI收割
来源：兰州化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。