Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I
文献类型：期刊论文
| 作者 | Chen, JJ ; Zhang, C ; Zhao, BY ; Mi, LJ（米丽娟） ; Zhang, Y(张益） ; Hu, J（胡钧） |
| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
 |
| 出版日期 | 2012 |
| 卷号 | 23期号:4 |
| ISSN号 | 1001-8042 |
| 英文摘要 | Fluorescence enhancement in a DNA-dye system is favorable for sensitive and accurate DNA detection/sensing technologies. In this paper, we report that the fluorescence of the double-stranded DNA (dsDNA) and SYBR Green I (SG) system (dsDNA-SG) can be effectively enhanced by negatively charged magnetic iron oxide (Fe2O3@DMSA) and gold nanoparticles in suitable concentrations, but positively charged nanoparticles quench the fluorescence. Effects of the Fe2O3@DMSA on the fluorescence intensities are investigated with dsDNA-SG of different lengths or complexities. The results show that nanoparticles perform similarly in enhancing fluorescence intensity for several kinds of dsDNA. However, the dsDNA concentration determines the fluorescence amplitude. It shows that fluorescence intensity of lower concentration dsDNA is enhanced remarkably in DNA-SG. The finding may be useful in sensitive biomolecular detection. |
| 语种 | 英语 |
| WOS记录号 | WOS:000310092200011 |
| 公开日期 | 2013-12-05 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13143]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Chen, JJ,Zhang, C,Zhao, BY,et al. Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I[J]. NUCLEAR SCIENCE AND TECHNIQUES,2012,23(4). |
| APA | Chen, JJ,Zhang, C,Zhao, BY,Mi, LJ,Zhang, Y,&Hu, J.(2012).Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I.NUCLEAR SCIENCE AND TECHNIQUES,23(4). |
| MLA | Chen, JJ,et al."Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I".NUCLEAR SCIENCE AND TECHNIQUES 23.4(2012). |
入库方式： OAI收割
来源：上海应用物理研究所
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