Genetic analysis with nanoPCR
文献类型：期刊论文
| 作者 | Pan, D(潘敦) ; Mi, LJ(米丽娟) ; Huang, Q(黄庆) ; Hu, J(胡钧) ; Fan, CH(樊春海) |
| 刊名 | INTEGRATIVE BIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 4期号:10 |
| ISSN号 | 1757-9694 |
| 英文摘要 | Polymerase chain reaction (PCR) has become a standard and important molecular biological technique with numerous applications in genetic analysis, forensics and in vitro diagnostics. Since its invention in the 1980s, there has been dramatic performance improvement arising from long-lasting efforts to optimize amplification conditions in both academic studies and commercial applications. More recently, a range of nanometer-sized materials including metal nanoparticles, semiconductor quantum dots, carbon nanomaterials and polymer nanoparticles, have shown unique effects in tuning amplification processes of PCR. It is proposed that these artificial nanomaterials mimic protein components in the natural DNA replication machinery in vivo. These so-called nanomaterials-assisted PCR (nanoPCR) strategies shed new light on powerful PCR with unprecedented sensitivity, selectivity and extension rate. In this review, we aim to summarize recent progress in this direction and discuss possible mechanisms for such performance improvement and potential applications in genetic analysis (particularly gene typing and haplotyping) and diagnostics. |
| 语种 | 英语 |
| WOS记录号 | WOS:000309127600001 |
| 公开日期 | 2013-12-05 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13174]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Pan, D,Mi, LJ,Huang, Q,et al. Genetic analysis with nanoPCR[J]. INTEGRATIVE BIOLOGY,2012,4(10). |
| APA | Pan, D,Mi, LJ,Huang, Q,Hu, J,&Fan, CH.(2012).Genetic analysis with nanoPCR.INTEGRATIVE BIOLOGY,4(10). |
| MLA | Pan, D,et al."Genetic analysis with nanoPCR".INTEGRATIVE BIOLOGY 4.10(2012). |
入库方式： OAI收割
来源：上海应用物理研究所
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