Local and global sensitivity analysis of PCR kinetic model
文献类型：期刊论文
| 作者 | Li, LT（李兰婷） ; Mi, LJ（米丽娟） ; Hu, J（胡钧） |
| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
 |
| 出版日期 | 2012 |
| 卷号 | 23期号:2 |
| ISSN号 | 1001-8042 |
| 英文摘要 | A mathematical model of polymerase chain reaction (PCR) containing uncertain and time-varying parameters has become important for model-based guidance experiment design. In this study, the local and global sensitivity analyses were conducted to identify that the responses of PCR process vary with their parameters of initial reactant concentrations and rate constants. Our results showed that the template concentration in initial reactant concentrations had the largest effect on DNA amplification yield. The rate constant characteristics showed that the local sensitivity basically determined the specific reactions; and the global sensitivity, the non-specific reactions. Our work should be helpful for optimizing PCR experimental conditions, and determining the PCR parameter sensitivities. |
| 语种 | 英语 |
| WOS记录号 | WOS:000303784600011 |
| 公开日期 | 2013-12-05 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13210]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Li, LT,Mi, LJ,Hu, J. Local and global sensitivity analysis of PCR kinetic model[J]. NUCLEAR SCIENCE AND TECHNIQUES,2012,23(2). |
| APA | Li, LT,Mi, LJ,&Hu, J.(2012).Local and global sensitivity analysis of PCR kinetic model.NUCLEAR SCIENCE AND TECHNIQUES,23(2). |
| MLA | Li, LT,et al."Local and global sensitivity analysis of PCR kinetic model".NUCLEAR SCIENCE AND TECHNIQUES 23.2(2012). |
入库方式： OAI收割
来源：上海应用物理研究所
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