X-ray Phase Contrast Imaging of Cell Isolation with Super-Paramagnetic Microbeads
文献类型：期刊论文
| 作者 | Tang, RB ; Chai, WM ; Yang, GY ; Xie, HL（谢红兰） ; Chen, KM |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:9 |
| ISSN号 | 1932-6203 |
| 英文摘要 | Super-paramagnetic microbeads are widely used for cell isolation. Evaluation of the binding affinity of microbeads to cells using optical microscopy has been limited by its small scope. Here, magnetic property of microbeads was first investigated by using synchrotron radiation (SR) in-line x-ray phase contrast imaging (PCI). The cell line mouse LLC (Lewis lung carcinoma) was selected for cell adhesion studies. Targeted microbeads were prepared by attaching anti-VEGFR2 (vascular endothelial growth factor receptor-2) antibody to the shell of the microbeads. The bound microbeads were found to better adhere to LLC cells than unbound ones. PCI dynamically and clearly showed the magnetization and demagnetization of microbeads in PE-50 tube. The cells incubated with different types of microbeads were imaged by PCI, which provided clear and real-time visualization of the cell isolation. Therefore, PCI might be considered as a novel and efficient tool for further cell isolation studies. |
| 语种 | 英语 |
| WOS记录号 | WOS:000309554700048 |
| 公开日期 | 2013-12-05 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13345]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Tang, RB,Chai, WM,Yang, GY,et al. X-ray Phase Contrast Imaging of Cell Isolation with Super-Paramagnetic Microbeads[J]. PLOS ONE,2012,7(9). |
| APA | Tang, RB,Chai, WM,Yang, GY,Xie, HL,&Chen, KM.(2012).X-ray Phase Contrast Imaging of Cell Isolation with Super-Paramagnetic Microbeads.PLOS ONE,7(9). |
| MLA | Tang, RB,et al."X-ray Phase Contrast Imaging of Cell Isolation with Super-Paramagnetic Microbeads".PLOS ONE 7.9(2012). |
入库方式： OAI收割
来源：上海应用物理研究所
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