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Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge
文献类型：期刊论文
| 作者 | Wang, ZL ; Wu, ZH ; Jian, JC ; Lu, YS |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 26期号:4页码:639-645 |
| 关键词 | SSH EST RACE Bacterial challenge Real-time PCR |
| ISSN号 | 1050-4648 |
| 通讯作者 | wuzh@gdou.edu.cn |
| 中文摘要 | The cDNA of pearl oyster Pinctada fucata Hsp70 (designated PFHsp70) was cloned by EST and rapid amplification of cDNA ends (RACE) techniques. The full length of PFHsp70 cDNA was 2376 bp, consisting of a 5'-terminal untranslated region (UTR) of 89 bp, a 3' terminal UTR of 328 bp, and an open reading frame (ORF) of 1959 bp encoding a polypeptide of 652 amino acids with a theoretical molecular weight of 71.42 kDa and an estimated isoelectric point of 5.18. BLAST analysis revealed that the PFHsp70 gene shared high similarity with other Hsp70 genes. PFHsp70 contained all the three classical Hsp70 family signatures. The results indicated that the PFHsp70 was a member of the heat shock protein 70 family. Fluorescent real-time quantitative RT-PCR was used to examine the expression of PFHsp70 gene in haemocytes of P. fucata after the challenge of bacteria Vibrio alginolyticus. There was a clear time-dependent expression pattern of PFHsp70 after bacterial challenge, and the mRNA expression reached a maximum level at 4 h post-challenge, which returned to control level after 32 h. The up-regulated mRNA expression of PFHsp70 in P. fucata after bacterial challenge indicates that the Hsp70 gene is inducible and involved in immune response. (C) 2008 Published by Elsevier Ltd. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 收录类别 | SCI |
| 原文出处 | ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000266194500010 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5114]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, ZL,Wu, ZH,Jian, JC,et al. Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(4):639-645. |
| APA | Wang, ZL,Wu, ZH,Jian, JC,&Lu, YS.(2009).Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge.FISH & SHELLFISH IMMUNOLOGY,26(4),639-645. |
| MLA | Wang, ZL,et al."Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge".FISH & SHELLFISH IMMUNOLOGY 26.4(2009):639-645. |
入库方式： OAI收割
来源：南海海洋研究所
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