Phenotypic and genetic differences between opaque and translucent colonies of Vibrio alginolyticus
文献类型：期刊论文
| 作者 | Chen, C ; Xie, J ; Hu, CQ |
| 刊名 | BIOFOULING
 |
| 出版日期 | 2009 |
| 卷号 | 25期号:6页码:525-531 |
| 关键词 | colony morphology phase variation biofilm polysaccharide Vibrio alginolyticus motility |
| ISSN号 | 0892-7014 |
| 通讯作者 | chen.chang@scsio.ac.cn |
| 中文摘要 | Many pathogens undergo phase variation between rugose and smooth colony morphology or between opaque and translucent colony morphology, which is mainly due to the variation in the surface polysaccharides. In this study, Vibrio alginolyticus ZJ-51 displayed phase variation between opaque, rugose colonies (Op) and translucent, smooth colonies (Tr). Unlike the vibrios reported previously, Tr cells of ZJ-51 enhanced biofilm formation and motility, but they did not differ from Op cells in the quantity of surface polysaccharides produced. Real time PCR was used to analyze the expression of the genes involved in polysaccharide biosynthesis, flagellar synthesis, and the AI-2 quorum-sensing system. The results revealed that the K-antigen capsule gene cluster (which consists of homologs to the cpsA-K in Vibrio parahaemolyticus) and O-antigen polysaccharide gene cluster (which contains homologs to the wza-wzb-wzc) were significantly more transcribed in Tr cells. The AI-2 quorum-sensing genes showed enhanced expression in the Tr variant which also exhibited greater expression of genes associated with polar flagellar biosynthesis. These results suggest that colony phase variation might affect the virulence and survival ability in the stressful environment inhabited by V. alginolyticus. |
| 学科主题 | Biotechnology & Applied Microbiology; Marine & Freshwater Biology |
| 收录类别 | SCI |
| 原文出处 | TAYLOR & FRANCIS LTD |
| WOS记录号 | WOS:000265633300005 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5120]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Chen, C,Xie, J,Hu, CQ. Phenotypic and genetic differences between opaque and translucent colonies of Vibrio alginolyticus[J]. BIOFOULING,2009,25(6):525-531. |
| APA | Chen, C,Xie, J,&Hu, CQ.(2009).Phenotypic and genetic differences between opaque and translucent colonies of Vibrio alginolyticus.BIOFOULING,25(6),525-531. |
| MLA | Chen, C,et al."Phenotypic and genetic differences between opaque and translucent colonies of Vibrio alginolyticus".BIOFOULING 25.6(2009):525-531. |
入库方式： OAI收割
来源：南海海洋研究所
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