Sensitive Determination of Saponins in Radix et Rhizoma Notoginseng by Charged Aerosol Detector Coupled with HPLC
文献类型：期刊论文
| 作者 | Bai, CC ; Han, SY ; Chai, XY ; Jiang, Y ; Li, P ; Tu, PF |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2009 |
| 卷号 | 32期号:2页码:242-260 |
| 关键词 | Charged aerosol detector evaporative light scattering detector HPLC Panax notoginseng Radix et Rhizoma Notoginseng saponins |
| ISSN号 | 1082-6076 |
| 通讯作者 | pengfeitu@vip.163.com |
| 中文摘要 | With continuous development in analytical instruments over recent years, high performance liquid chromatography ( HPLC) with various detectors has become very important tools for routine analysis and quality control of TCMs and botanical medicines. As a new "mass'' detector, charged aerosol detector ( CAD) provides an alternative detection method, which ultraviolet (UV) is unable to achieve for components owning almost no chromophore in its chemical structures with the lower sensitivity. With the purpose of evaluating the applicability of CAD for the analysis of TCMs, an HPLC-CAD method for simultaneous determination of saponins in Radix et Rhizoma Notoginseng ("Sanqi'' in chinese) was established in this contribution, which was successfully applied for quantitation of seven saponins, notoginsenoside R-1, ginsenosides Rg(1), Re, Rb-1, Rg(2), Rh-1, and Rd, in thirty batches of Sanqi samples. Meanwhile, the LODs and LOQs of UV, ELSD and CAD were compared and the results showed that the CAD method exhibited a lower LOD ( 0.01 similar to 0.15 mu g) and LOQ ( 0.04 similar to 0.41 mu g) than UV and ELSD. Furthermore, the CAD exhibited a steadier baseline in gradient elution compared with UV at 203 nm. Also, the HPLC-CAD method presents many apparent advantages of high sensitivity, steady baseline in gradient elution, easy operating, and it was expected to be a sensitive and universal method for analysis of TCMs containing weak UV absorption compounds. It was demonstrated that the content of seven saponins in Radix et Rhizoma Notoginseng did not significantly correlate with the original work nor with the conventional quality standard. |
| 学科主题 | Biochemical Research Methods; Chemistry, Analytical |
| 收录类别 | SCI |
| 原文出处 | TAYLOR & FRANCIS INC |
| WOS记录号 | WOS:000261875000006 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5156]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Bai, CC,Han, SY,Chai, XY,et al. Sensitive Determination of Saponins in Radix et Rhizoma Notoginseng by Charged Aerosol Detector Coupled with HPLC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2009,32(2):242-260. |
| APA | Bai, CC,Han, SY,Chai, XY,Jiang, Y,Li, P,&Tu, PF.(2009).Sensitive Determination of Saponins in Radix et Rhizoma Notoginseng by Charged Aerosol Detector Coupled with HPLC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,32(2),242-260. |
| MLA | Bai, CC,et al."Sensitive Determination of Saponins in Radix et Rhizoma Notoginseng by Charged Aerosol Detector Coupled with HPLC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 32.2(2009):242-260. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。