Variability of ribosomal DNA ITS-2 and its utility in detecting genetic relatedness of pearl oyster
文献类型：期刊论文
| 作者 | He, MX ; Huang, LM ; Shi, JH ; Jiang, YP |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 7期号:1页码:40-45 |
| 关键词 | pearl oyster internal transcribed spacer 2 variability phylogenetic analysis species identification |
| ISSN号 | 1436-2228 |
| 通讯作者 | hmx@scsio.ac.cn |
| 中文摘要 | The objective of this study was to detect interspecific and intraspecific genetic variations of the second internal transcribed spacer of ribosomal DNA (ITS-2), and explore the feasibility of using it as a molecular marker phylogenetic analyses and species identification among pearl oysters. ITS-2 sequences of 6 pearl oysters were amplified via polymerase chain reaction. The amplified DNA fragments were about 500 bp, spanning the partial sequences of 5.8S and 28S rRNA genes. The GC contents of all species used in this study were higher than the AT contents. The variations of sequences involved substitutions as well as insertions/deletions and were mainly concentrated in spacer regions. Sequences of about 30-bp in spacer regions showed no variations among 5 Pincatda species. Intraindividual and intraspecific polymorphisms of ITS-2 sequences were detected in some species; the interspecific variability was significantly larger than the variability within species, and the variability at the genus level was higher than that at the species level. Both neighbor-joining and parsimony analyses of ITS-2 sequences revealed the distinguishable species boundary of 6 pearl oysters, and indicated that P. chemnitzi and P. nigra were the closely related species, as were P. maxima and P. margaritifera. The findings revealed that ITS-2 sequences could be an appropriate tool for phylogenetic study of pearl oysters. |
| 学科主题 | Biotechnology & Applied Microbiology; Marine & Freshwater Biology |
| 收录类别 | SCI |
| WOS记录号 | WOS:000229540200006 |
| 公开日期 | 2011-06-29 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/2245]  |
| 专题 | 南海海洋研究所_广东省海洋药物重点实验室 |
| 推荐引用方式 GB/T 7714 | He, MX,Huang, LM,Shi, JH,et al. Variability of ribosomal DNA ITS-2 and its utility in detecting genetic relatedness of pearl oyster[J]. MARINE BIOTECHNOLOGY,2005,7(1):40-45. |
| APA | He, MX,Huang, LM,Shi, JH,&Jiang, YP.(2005).Variability of ribosomal DNA ITS-2 and its utility in detecting genetic relatedness of pearl oyster.MARINE BIOTECHNOLOGY,7(1),40-45. |
| MLA | He, MX,et al."Variability of ribosomal DNA ITS-2 and its utility in detecting genetic relatedness of pearl oyster".MARINE BIOTECHNOLOGY 7.1(2005):40-45. |
入库方式： OAI收割
来源：南海海洋研究所
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