Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Chen, F ; Li, HB ; Wong, RNS ; Ji, B ; Jiang, Y |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2005 |
| 卷号 | 1064期号:2页码:183-186 |
| 关键词 | Microcystis aeruginosa counter-current chromatography preparative chromatography zeaxanthin carotenoids |
| ISSN号 | 0021-9673 |
| 通讯作者 | sfchen@hkusua.hku.hk |
| 中文摘要 | High-speed counter-current chromatography was successfully applied for the first time to the isolation and purification of the bioactive carotenoid zeaxanthin from the cyanobacterium Microcystis aeruginosa. The crude zeaxanthin was obtained by extraction with organic solvents after the microalgal sample had been saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (8:2:7:3, v/v/v/v) was successfully performed yielding zeaxanthin at 96.2% purity from 150 mg of the crude extract in a one-step separation. The recovery of zeaxanthin was 91.4%. This was also the first report that zeaxanthin was successfully separated and purified from microalgae. (C) 2004 Published by Elsevier B.V. |
| 学科主题 | Biochemical Research Methods; Chemistry, Analytical |
| 收录类别 | SCI |
| WOS记录号 | WOS:000226782100005 |
| 公开日期 | 2011-06-29 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/2255]  |
| 专题 | 南海海洋研究所_广东省海洋药物重点实验室 |
| 推荐引用方式 GB/T 7714 | Chen, F,Li, HB,Wong, RNS,et al. Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1064(2):183-186. |
| APA | Chen, F,Li, HB,Wong, RNS,Ji, B,&Jiang, Y.(2005).Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1064(2),183-186. |
| MLA | Chen, F,et al."Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1064.2(2005):183-186. |
入库方式： OAI收割
来源：南海海洋研究所
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