Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis
文献类型：期刊论文
| 作者 | Wu, XZ ; Sun, JF ; Zhang, WZ ; Wen, BB |
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2005 |
| 卷号 | 67期号:40545页码:149-154 |
| 关键词 | rickettsia-like organism Crassostrea ariakensis antigenic characteristics purification |
| ISSN号 | 0177-5103 |
| 通讯作者 | wuxz@zju.edu.cn |
| 中文摘要 | A rickettsia-like organism (RLO) has been suggested to be the etiological agent responsible for heavy losses of the oyster Crassostrea ariakensis Gould in China. Because of the lack of molluscan cell lines for in vitro culture of intracellular prokaryotes, antigenic analysis of RLOs has been limited by the inherent difficulties of their purification. In this report, we describe the use of differential speed centrifugation and renografin density gradient centrifugation to purify the RLO directly from infected oyster tissues. The purity and integrity of purified prokaryotes were validated by transmission electron microscopy. Thirteen major constituent proteins, with molecular weights ranging between 17 and 99 kDa, were electrophoretically identified by silver staining, and 8 major proteins were identified with Coomassie blue R staining. Specific mouse polyclonal antiserum was prepared for serological characterization of the RLO and was used in an immunoblot assay, and 3 major antigen groups were identified. The present results advance our knowledge of RLO protein antigens, and several proteins have been identified that could potentially be useful for diagnostic assays or for production of experimental immunostimulants. |
| 学科主题 | Fisheries; Veterinary Sciences |
| 收录类别 | SCI |
| WOS记录号 | WOS:000234935200021 |
| 公开日期 | 2011-07-02 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/3946]  |
| 专题 | 南海海洋研究所_海洋生物 |
| 推荐引用方式 GB/T 7714 | Wu, XZ,Sun, JF,Zhang, WZ,et al. Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis[J]. DISEASES OF AQUATIC ORGANISMS,2005,67(40545):149-154. |
| APA | Wu, XZ,Sun, JF,Zhang, WZ,&Wen, BB.(2005).Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis.DISEASES OF AQUATIC ORGANISMS,67(40545),149-154. |
| MLA | Wu, XZ,et al."Purification and antigenic characteristics of a rickettsia-like organism from the oyster Crassostrea ariakensis".DISEASES OF AQUATIC ORGANISMS 67.40545(2005):149-154. |
入库方式： OAI收割
来源：南海海洋研究所
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